Molecular phylogeny of a cosmopolitan group of woodpeckers (genus Picoides) gased on COI and cyt b mitochondrial gene sequences

Abstract

Picoides is the largest genus of woodpeckers and member species are found on most major land masses. Current systematic arrangement of this group, based on morphological, behavioral, and plumage characters, suggests that New World species evolved from a single invasion by a Eurasian common ancestor and that all New World species form a monophyletic group. No clear link has ever been established between the relationships of Old World and New World species other than to infer that the most primitive species is Eurasian. This study employs DNA sequences for two protein-coding mitochondrial genes, cytochrome oxidase I and cytochrome b, to reconstruct phylogenetic relationships among all New World species and several Eurasian representatives of the genus Picoides. A well-resolved mitochondrial gene tree is in direct conflict with proposed species relationships based on nongenetic characters; monophyly among New World species is rejected, the evolution of New World species likely resulted from as many as three independent Eurasian invasions, and Picoides is paraphyletic with two other woodpecker genera, Veniliornis and Dendropicos. These results strongly suggest that this large, cosmopolitan genus is in need of systematic revision in order to reflect evolutionary history.