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. 1975 Oct;81(1):1-14.

Volume changes induced in rabbit polymorphonuclear leukocytes by chemotactic factor and cytochalasin B

L S Hsu, E L Becker

Volume changes induced in rabbit polymorphonuclear leukocytes by chemotactic factor and cytochalasin B

L S Hsu et al. Am J Pathol. 1975 Oct.

Abstract

Incubation of rabbit neutrophils with various chemotactic factors causes an expansion of their volume. The expansion shows a high correlation with the chemotactic responsiveness of these cells, requires metabolic energy, and is independent of the presence of divalent cations. Cytochalasin B causes a decrease in the volume of the neutrophil. This decrease also requires metabolic energy and is independent of divalent cations. In the presence of cytochalasin B, the chemotactic factor, instead of acting to expand cell volume, induces a further contraction of the cell; this decrease requires Ca2+ in the external medium.

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