Mitosis and motor proteins in the filamentous ascomycete, Nectria haematococca, and some related fungi

Abstract

Among filamentous fungi, mitosis has been studied in-depth in just a few species. The mitotic apparatuses in the ascomycetous Fusarium spp. are the most clearly and readily visualized in vivo within this group; fluorescent labeling is unnecessary. This superior cytological tractability has enabled detailed studies and revealing experiments that have led the way toward a more complete understanding of fungal mitosis. Some of the most important discoveries include the role of half-spindles in development of the bipolar spindle, the existence of true kinetochores in fungi, the unorthodox chromosome configurations and movements comprising metaphase and anaphase A, the attachment of astral microtubules to the plasmalemma, the role of the astral pulling force in elongating the spindle, an inwardly directed force within the spindle, and microtubule cross-bridging in both spindle and asters. Recent research has focused on the roles of microtubuleassociated motor proteins in Fusarium solani f. sp. pisi (anamorph of Nectria haematococca). Cytoplasmic dynein was shown to be involved in the development and/or maintenance of mitotic asters and necessary for motility and functionality of the interphase spindle pole body. The inwardly directed force within the anaphase spindle was shown to be produced by a kinesin-related protein, NhKRP1. Because of its superior cytological tractability, the considerable and unique knowledge we have of many aspects of its mitosis, and its genetic tractability, Fusarium solani f. sp. pisi is a good choice for further investigations of mitosis in filamentous fungi.