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. 2001 Dec;26(5):635-40.
doi: 10.1007/BF02704761.

Recombinant pre-pro-Concanavalin A (jack bean) is stable but of low solubility

H B Dincturk  1 , A J Dunn, D H Jones
Affiliations

Recombinant pre-pro-Concanavalin A (jack bean) is stable but of low solubility

H B Dincturk et al. J Biosci. 2001 Dec.

Abstract

The cDNA for pre-pro-Concanavalin A (pre-pro-ConA) was cloned into the cytoplasmic expression vector pKK233-2 to give rise to pCONEXP2 which was used to express the lectin precursor. Pre-pro-ConA is stable and is not transposed and ligated to form the mature protein. No signal peptide removal is observed. The solubility of pre-pro-ConA could not be increased by guanidine hydrochloride denaturation/dilution treatment.

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