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. 2001 Dec;18(12):623-8.
doi: 10.1023/a:1013171505702.

A prospective study on oocyte survival rate after ICSI: influence of injection technique and morphological features

T Ebner  1 , C Yaman, M Moser, M Sommergruber, K Jesacher, G Tews
Affiliations

A prospective study on oocyte survival rate after ICSI: influence of injection technique and morphological features

T Ebner et al. J Assist Reprod Genet. 2001 Dec.

Abstract

Purpose: To determine the influence of technical pitfalls and oocyte morphology on survival rate and cleavage behavior after ICSI.

Methods: A total of 2210 injection procedures was examined for morphological and technical deviations. Survival rate and cleavage behavior were evaluated.

Results: In 77.8% of all cases ICSI was unsuspicous. Out of 491 deviations from optimal injection deep penetration of the oocyte and abundant presence of cumulus cells showed significant correlation with degeneration rate (p < 0.001). Morphological anomalies associated with the periphery of the oocyte were rather related to degeneration than cytoplasmic anomalies (p < 0.001). Early embryonic development was not impaired by technical or morphological parameters.

Conclusions: To conclude, these prospective data may be of prognostic value in regard of the number of embryos available for transfer and may help to improve treatment outcome.

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