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. 2001 Dec 15;29(24):E120.
doi: 10.1093/nar/29.24.e120.

A unique type II restriction endonuclease FspAI, that recognizes the octanucleotide sequence 5'-RTGC/GCAY-3'

A Kesminiene  1 , Z Maneliene, J Vitkute, M Petrusyte, A Janulaitis
Affiliations

A unique type II restriction endonuclease FspAI, that recognizes the octanucleotide sequence 5'-RTGC/GCAY-3'

A Kesminiene et al. Nucleic Acids Res. .

Abstract

A new type II restriction endonuclease designated FspAI has been partially purified from a Flexibacter species Tv-m21K. FspAI recognizes the octanucleotide sequence 5'-RTGC/GCAY-3' and cleaves it in the center generating blunt-ended DNA fragments.

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Figures

Figure 1
Figure 1
(A) Fragmentation of various DNA with FspAI. Lane 1, GeneRuler™ DNA Ladder Mix as size markers; lane 2, adenovirus-2 DNA + FspAI; lane 3, T7 DNA + FspAI; lane 4, pBR322 DNA + FspAI; lane 5, φX174 DNA + FspAI; lane 6, pUC19 DNA + FspAI; lane 7, M13mp18 DNA + FspAI; lane 8, λ DNA-Eco130I digest as size markers. (B) Double digestion of pBR322 DNA with FspAI and NsbI. Lane 1, pBR322 DNA; lane 2, pBR322 DNA + FspAI; lane 3, pBR322 DNA + FspAI + NsbI; lane 4, pBR322 DNA + NsbI.
Figure 1
Figure 1
(A) Fragmentation of various DNA with FspAI. Lane 1, GeneRuler™ DNA Ladder Mix as size markers; lane 2, adenovirus-2 DNA + FspAI; lane 3, T7 DNA + FspAI; lane 4, pBR322 DNA + FspAI; lane 5, φX174 DNA + FspAI; lane 6, pUC19 DNA + FspAI; lane 7, M13mp18 DNA + FspAI; lane 8, λ DNA-Eco130I digest as size markers. (B) Double digestion of pBR322 DNA with FspAI and NsbI. Lane 1, pBR322 DNA; lane 2, pBR322 DNA + FspAI; lane 3, pBR322 DNA + FspAI + NsbI; lane 4, pBR322 DNA + NsbI.
Figure 2
Figure 2
Determination of the FspAI cleavage position. Lanes G, A, T and C, the sequence ladders; lane 1, the product of the primed synthesis reaction cleaved with FspAI; lane 2, the T4 DNA polymerase action on the FspAI digest. The nucleotides of the FspAI site are shown on the left.
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