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. 1998 Apr;4(2):140-143.
doi: 10.3748/wjg.v4.i2.140.

Evaluation of various solutions for small bowel graft preservation

You-Sheng Li, Jie-Shou Li, Ning Li, Zhi-Wei Jiang, Yun-Zhao Zhao, Nan-Yun Li, Fang-Nan Liu

Evaluation of various solutions for small bowel graft preservation

You-Sheng Li et al. World J Gastroenterol. 1998 Apr.

Abstract

AIM:To elucidate the effect of various solutions for small bowel graft preservation in pigs under hypothermic storage.METHODS:The swine segmental small bowel graft was autotransplanted after it was preserved with lactated Ringer's (LR), Euro-Collins (EC), hyperosmolarity citrate adenine (HC-A) and WMO-1 solutions for 10,18 and 24 hours,respectively.The recipient survival rate, morphological structure, graft mucosal energy substances and Na( +) -K(+) ATPase activity were studied,and graft absorption was estimated with D-xylose absorption test.RESULTS:The morphological study of the grafts preserved with LR or HC-A solution for 10 hours or with EC and WMO-1 solution for 18 hours was normal 6days after operation. Mucosal ATP,total adenine nucleotides (TAN) contents and Na( +) -K(+)ATPase activity of the graft preserved with EC or WMO solution were higher than that of the graft preserved with LR or HC-A solution.Serum level of D-xylose was higher in EC and WMO-1 groups than in LR and HC-A groups when the graft was preserved for 24 hours.CONCLUSION:EC and WMO-1 solutions can preserve the swine small bowel up to 18 hours, which are superior to LR and HC-A solutions.

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Figures

Figure 1
Figure 1
Mucosal concentration of ATP.
Figure 2
Figure 2
Mucosal concentration of TNA.
Figure 3
Figure 3
Results of D-xylose absorption test after 24 h of cold storage.
See this image and copyright information in PMC

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