DNA and DNA polymerase in the core of the Dane particle of hepatitis B

Abstract

The core of the Dane particle was shown to contain a DNA polymerase and a circular double stranded DNA with a molecular weight of 1.6 X 10(6) daltons which served as the primer-template for the enzyme. The product of the DNA polymerase reaction was in a base paired form and was covalently attached to the circular DNA. Neither the circular DNA nor the attached DNA product of the enzyme reaction was attacked by the DNase or released from intact cores until the cores were disrupted with sodium dodecyl sulfate, suggesting that they are internal components of the core. The DNA polymerase is a specific marker for Dane particles and can be used to distinguish sera with high and low concentrations of Dane particles. The DNA polymerase reaction can also be used to radiolabel Dane particle cores for a specific and sensitive radioimmunoprecipitation assay for antibody against the hepatitis B core antigen (anti-HBc).