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. 1999 Aug;5(4):324-326.
doi: 10.3748/wjg.v5.i4.324.

Codon 249 mutations of p53 gene in non-neoplastic liver tissues

Xiao-Mou Peng, Chun-Lan Yao, Xue-Juan Chen, Wen-Wei Peng, Zhi-Liang Gao

Codon 249 mutations of p53 gene in non-neoplastic liver tissues

Xiao-Mou Peng et al. World J Gastroenterol. 1999 Aug.

Abstract

AIM:To study the significance of p53 gene in hepatocarcinogenesis through analyzing codon 249 mutations of p53 gene in non-neoplastic liver tissues.METHODS:Codon 249 mutation was detected using single-stranded conformational polymorphism analysis and allele-specific PCR in liver tissues from 10 cases of chronic hepatitis, 5 cases of cirrhosis and 20 cases of HCCs.RESULTS:The detection rate of codon 249 mutation in chronic hepatitis, cirrhosis and pericancerous tissues was 70% (7/10), 100% (5/5) and 70% (14/20), respectively by AS-PCR. These mutations could not be detected by SSCP analysis. The detection rates were 65% (13/20) and 45% (9/20) in cancerous tissues by AS-PCR and SSCP analysis.CONCLUSION:Codon 249 mutations of p53 gene were very popular in non-neoplastic liver tissues though the number of those mutant cells was only in subsection. Those mutations in cancerous tissues might take place in the stage before the formation of tumor.

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Figures

Figure 1
Figure 1
PCR/SSCP analysis of HCC samples. CT represents cancerous tissue, PCT represents pericancerous tissue, shifted bands were marked by arrows. Silver staining.
Figure 2
Figure 2
AS-PCR products were analyzed by running on 2% agarose gel and visualized by staining with ethidium bromide. Lane 1 was 100 bp ladder from GIBCO, Gaithersburg, Md, USA; Lane 2, 4, 5, 7 was positive; Lane 3, 6 are negative.
See this image and copyright information in PMC

References

    1. Hollstein M, Sidransky D, Vogelstein B, Harris CC. p53 mutations in human cancers. Science. 1991;253:49–53. - PubMed
    1. Hsu IC, Metcalf RA, Sun T, Welsh JA, Wang NJ, Harris CC. Mutational hotspot in the p53 gene in human hepatocellular carcinomas. Nature. 1991;350:427–428. - PubMed
    1. Bressac B, Kew M, Wands J, Ozturk M. Selective G to T mutations of p53 gene in hepatocellular carcinoma from southern Africa. Nature. 1991;350:429–431. - PubMed
    1. Peng XM, Peng WW, Zhou YP. Studies on mutations of p53 gene in HCCs. Zhongshan Yike Daxue Xuebao. 1997;18:245–248.
    1. Slagle BL. p53 mutations and hepatitis B virus: cofactors in hepatocellular carcinoma. Hepatology. 1995;21:597–599. - PubMed