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. 2000 Jun;6(3):353-355.
doi: 10.3748/wjg.v6.i3.353.

Effect of intestinal ischemia-reperfusion on expressions of endogenous basic fibroblast growth factor and transforming growth factor betain lung and its relation with lung repair

Xiao-Bing Fu, Yin-Hui Yang, Tong-Zhu Sun, Xiao-Man Gu, Li-Xian Jiang, Xiao-Qing Sun, Zhi-Yong Sheng

Effect of intestinal ischemia-reperfusion on expressions of endogenous basic fibroblast growth factor and transforming growth factor betain lung and its relation with lung repair

Xiao-Bing Fu et al. World J Gastroenterol. 2000 Jun.

Abstract

AIM:To study the changes of endogenous transforming growth factor beta(TGFbeta) and basic fibroblast growth factor (bFGF) in lung following intestinal ischemia and reperfusion injury and their effects on lung injury and repair.METHODS:Sixty Wistar rats were divided into five groups, which underwent sham-operation, ischemia (45 minutes), and reperfusion (6, 24 and 48 hours, respectively) after ischemia (45 minutes). Immunohistochemical method was used to observe the localization and amounts of both growth factors.RESULTS:Positive signals of both growth factors could be found in normal lung, mainly in alveolar cells and endothelial cells of vein. After ischemia and reperfusion insult, expressions of both growth factors were increased and their amounts at 6 hours were larger than those of normal control or of 24 and 48 hours after insult.CONCLUSION:The endogenous bFGF and TGF beta expression appears to be upregulated in the lung following intestinal ischemia and reperfusion, suggesting that both growth factors may be involved in the process of lung injury and repair.

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Figures

Figure 1
Figure 1
The expressions of bFGF (A) and TGF-β (B) in normal lung. The weakly positive signal could be found in alveolar epithelial cells and microvascular endothelial cells. SP stain × 400
Figure 2
Figure 2
The expressions of bFGF (A) and TGF-β (B) in damaged lung following ischemia (45 min) and reperfusion (6 h). Deep staining of both growth factors could be found in alveolar epithelial cells and microvascular endothelial cells. SP stain × 400
Figure 3
Figure 3
The expressions of bFGF (A) and TGF-β (B) in damaged lung following ischemia (45 min) and reperfusion (24 h). The staining of both growth factors could be found in alveolar epithelial cells and microvascular endothelial cells and the expression returned to normal levels. SP stain × 400
See this image and copyright information in PMC

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