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. 2000 Oct;6(5):676-680.
doi: 10.3748/wjg.v6.i5.676.

Effects of tachyplesin on the morphology and ultrastructure of human gastric carcinoma cell line BGC-823

Qi-Fu Li, Gao-Liang Ou Yang, Chang-You Li, Shui-Gen Hong

Effects of tachyplesin on the morphology and ultrastructure of human gastric carcinoma cell line BGC-823

Qi-Fu Li et al. World J Gastroenterol. 2000 Oct.

Abstract

AIM:To investigate the morphological and ultrastructural changes in the human gastric carcinoma cell line BGC-823 after being treated with tachyplesin.METHODS:Tachyplesin was isolated from acid extracts of Chinese horseshoe crab (Tachypleus tridentatus) hemocytes. BGC-823 cells and the cells treated with 2.0mg/L tachyplesin were examined respectively under light microscope, scanning and transmission electron microscope.RESULTS: BGC-823 cells had undergone the restorational alteration in morphology and ultrastructure after tachyplesin treatment. The changes were as follows: the shape of cells was unanimous, the volume enlarged and cells turned to be flat and spread, the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular, the number of nucleolus reduced and its volume lessened,heter-chromatin decreased while euchromatin increased in nucleus. In the cytoplasm, mitochondria grew in number with consistent structure relatively, Golgi complex turned to be typical and well-developed,rough endoplasmic reticulum increased and polyribosome decreased. The microvilli at cellular surface were rare and the filopodia reduced while lamellipodia increased at the cell edge.CONCLUSION:Tachyplesin could alter the malignant morphological and ultrastructural charact-eristics of human gastric carcinoma cells effectively and have a certain inducing differen-tiation effect on human gastric carcinoma cells.

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Figures

Figure 1
Figure 1
BGC-823 cells. × 300
Figure 2
Figure 2
BGC-823 cells treated with tachyplesin. × 300
Figure 3
Figure 3
There are abundant microvilli on spherical cell surface and many filopodia emerge at the edge of BGC-823 cells. × 6000
Figure 4
Figure 4
The microvilli on spherical cell surface disappear mainly in the cells after tachyplesin treatment. × 6000
Figure 5
Figure 5
The microvilli are plentiful on flat and spread cell surface and many filopodia appear at the edge of BGC-823 cells. × 4800
Figure 6
Figure 6
On the cells treated with tachyplsin the microvilli are rare, shrink and shortened on flat and spread cell surface and large lame llipodia emerge at cell edge. × 4200
Figure 7
Figure 7
The nucleo-cytoplasm ratio is large, the shape of nucleus (N) is irregular and only a few organelle in cytoplasm of BGC-823 cells (Nu nucleolus, M mitochordria, ER endoplasmic reticulum). × 9600
Figure 8
Figure 8
The shape and structure of mitochondria (M) is non-typical and endoplasmic reticulum (ER) decrease in BGC-823 cells. × 40500
Figure 9
Figure 9
The cellular volume enlarge, the nucleo-cytoplasm ratio decreases and the organelle increase in the cells treated with tachyplesin. × 10720
Figure 10
Figure 10
The shape and structure of mitochondria (M) are unanimity relatively and their cristae increase and arrange rather regularly in the cells treated with tachyplsin. × 21000
Figure 11
Figure 11
The structure of Golgi complex (G) appear typical and well-developed in the cells treated with tachyplsin. × 48000
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