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. 2001 Aug;7(4):551-4.
doi: 10.3748/wjg.v7.i4.551.

Differential expression of a novel colorectal cancer differentiation-related gene in colorectal cancer

X G Li  1 , J D Song, Y Q Wang
Affiliations

Differential expression of a novel colorectal cancer differentiation-related gene in colorectal cancer

X G Li et al. World J Gastroenterol. 2001 Aug.

Abstract

Aim: To investigate SBA2 expression in CRC cell lines and surgical specimens of CRC and autologous healthy mucosa.

Methods: Reverse transcription-polymerase chain reaction (RT-PCR) was used for relative quantification of SBA2 mRNA levels in 4 human CRC cell lines with different grades of differentiation and 30 clinical samples. Normalization of the results was achieved by simultaneous amplification of beta-actin as an internal control.

Results: In the exponential range of amplification, fairly good linearity demonstrated identical amplification efficiency for SBA2 and beta-actin (82%). Markedly lower levels of SBA2 mRNA were detectable in tumors, as compared with the coupled normal counterparts P<0.01). SBA2 expression was significantly (0.01>P < 0.05) correlated with the grade of differentiation in CRC, with relatively higher levels in well-differentiated samples and lower in poorly-differentiated cases. Of the 9 cases with lymph nodes affected, 78% (7/9) had reduced SBA2 mRNA expression in contrast to 24% (5/21) in non-metastasis samples 0.01>P<0.05).

Conclusion: SBA2 gene might be a promising novel biomarker of cell differentiation in colorectal cancer and its biological features need further studies.

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Figures

Figure 1
Figure 1
Detection of the exponential range by termination at se-quential PCR cycles. 1-7: Cycles of 20, 22, 24, 25, 26, 28 and 30, respectively. M: Marker (100 bp DNA ladder).
Figure 2
Figure 2
Detection of the exponential range by serial dilutions of cDNA. 1-4: cDNA of volume of 5, 2.5, 1.25, and 0.625 μL, respectively. M: Marker (100 bp DNA ladder).
Figure 3
Figure 3
Relative quantification of SBA2 mRNA in colorectal tissues. 1-4: Moderately, moderately, poorly, and well differentiated CRC, respectively. 5: Healthy colorectal mucosa. M: Marker (100 bp DNA ladder).
Figure 4
Figure 4
Relative quantification of SBA2 mRNA in cell lines of CRC. 1-4: Human CRC cell line CloneA, CX1, CCL187, and LS174T, respectively. M: Marker (100 bp DNA ladder).
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