Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2001 Oct;7(5):630-6.
doi: 10.3748/wjg.v7.i5.630.

Establishment of cell clones with different metastatic potential from the metastatic hepatocellular carcinoma cell line MHCC97

Y Li  1 , Z Y Tang, S L Ye, Y K Liu, J Chen, Q Xue, J Chen, D M Gao, W H Bao
Affiliations

Establishment of cell clones with different metastatic potential from the metastatic hepatocellular carcinoma cell line MHCC97

Y Li et al. World J Gastroenterol. 2001 Oct.

Abstract

Aim: To establish clone cells with different metastatic potential for the study of metastasis-related mechanisms.

Methods: Cloning procedure was performed on parental hepatocellular carcinoma (HCC) cell line MHCC97, and biological characteristics of the target clones selected by in vivo screening were studied.

Results: Two clones with high (MHCC97-H) and low (MHCC97-L) metastatic potential were isolated from the parent cell line. Compared with MHCC97-L, MHCC97-H had smaller cell size (average cell diameter 43 microm vs 50 microm) and faster in vitro and in vivo growth rate (tumor cell doubling time was 34.2h vs 60.0h). The main ranges of chromosomes were 55-58 in MHCC97-H and 57-62 in MHCC97-L. Boyden chamber in vitro invasion assay demonstrated that the number of penetrating cells through the artificial basement membrane was (37.5 +/- 11.0) cells/field for MHCC97-H vs (17.7 +/- 6.3)/field for MHCC97-L. The proportions of cells in G0-G1 phase, S phase, and G2-M phase for MHCC97-H/MHCC97-L were 0.56/0.65, 0.28/0.25 and 0.16/0.10, respectively, as measured by flow cytometry. The serum AFP levels in nude mice 5wk after orthotopic implantation of tumor tissue were (246 +/- 66) microg.L(-1) for MHCC97-H and (91 +/- 66) microg.L(-1) for MHCC97-L. The pulmonary metastatic rate was 100% (10/10) vs 40% (4/10).

Conclusion: Two clones of the same genetic background but with different biological behaviors were established, which could be valuable models for investigation on HCC metastasis.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Subcutaneous tumor formation of the two clones. Thirty days after sc injection of 5 × 106 tumor cells for each nude mouse, the average s.c tumor diameter was (1.94 ± 0.36) cm for MHCC97-H as against (0.84 ± 0.47) cm for MHCC97-L.
Figure 2
Figure 2
The liver tumor size of the two clones 5 wk after orthotopic inoculation. The tumor geometric mean diameter (GMD) for MHCC97-H was (1.42 ± 0.11) cm as against (0.90 ± 0.26) cm for MHCC97-L.
Figure 3
Figure 3
Photomicroscopy of lung metastases of the two clones. MHCC97-H (3A) produced large metastatic lesion pressing the bronchioles while MHCC97-L (3B) only formed small metastasis. HE × 100.
Figure 4
Figure 4
Photomicroscopy of MHCC97-L (4A) and MHCC97-H (4B) illustrating the clear differences in the number of nucleoli. Giemsa × 400.
Figure 5
Figure 5
Cell growth curve of two clones. 4 × 104 viable cells were cultured in each well of the 24 well culture plate. Cell numbers were determined for 7 consecutive days. Each time point represents the mean of duplicate cell counts.
See this image and copyright information in PMC

Comment in

References

    1. Fidler IJ. Tumor heterogeneity and the biology of cancer invasion and metastasis. Cancer Res. 1978;38:2651–2660. - PubMed
    1. Fidler IJ. Critical factors in the biology of human cancer metastasis: twenty-eighth G.H.A. Clowes memorial award lecture. Cancer Res. 1990;50:6130–6138. - PubMed
    1. Maekawa R, Kishimoto Y, Sato K, Homma M. Establishment of highly metastatic tumor clones derived from a skin squamous cell carcinoma (SqC-NH), D-1 and F-3, with distinct features of pulmonary metastases. Clin Exp Metastasis. 1993;11:343–353. - PubMed
    1. Navone NM, Olive M, Ozen M, Davis R, Troncoso P, Tu SM, Johnston D, Pollack A, Pathak S, von Eschenbach AC, et al. Establishment of two human prostate cancer cell lines derived from a single bone metastasis. Clin Cancer Res. 1997;3:2493–2500. - PubMed
    1. Dexter DL, Spremulli EN, Fligiel Z, Barbosa JA, Vogel R, VanVoorhees A, Calabresi P. Heterogeneity of cancer cells from a single human colon carcinoma. Am J Med. 1981;71:949–956. - PubMed

Publication types

MeSH terms