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. 1979 Dec;27(12):1634-7.
doi: 10.1177/27.12.118210.

Double labeling immunohistochemical technique provides evidence of the specificity of glial cell markers

M S Ghandour, O K Langley, G Vincendon, G Gombos

Double labeling immunohistochemical technique provides evidence of the specificity of glial cell markers

M S Ghandour et al. J Histochem Cytochem. 1979 Dec.

Abstract

Sequential treatment of rat cerebellar slices with two antisera, each specific for a different glial cell marker, revealed, by indirect immunofluroescence combined with the immunoperoxidase method on the same tissue section, that carbonic anhydrase isoenzyme II (CA II) is exclusively localized in oligodendrocytes while the glial fibrillary acidic protein (GFA) is, as well established, a specific marker for astrocytes.

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