Transport-associated phosphorylation of 2-deoxy-D-glucose in Saccharomyces fragilis

Abstract

2-Deoxy-D-glucose transport and metabolism was studied in Saccharomyces fragilis. Inside the cells four phosphorylated and three non-phosphorylated derivatives were found and identified. Accumulation of phosphorylated 2-deoxyglucose derivatives was balanced by a concomitant decrease of cellular ATP, orthophosphate and polyphosphates. The free sugar was concentrated against a concentration gradient, contradiciting facilitated diffusion. Pulse labeling experiments revealed transport-associated phosphorylation. Theoretical considerations and analysis of the effects of iodoacetate showed that an intracellular hexokinase activity was not involved in 2-deoxyglucose phosphorylation, although this sugar is a good substrate for the enzyme in in vitro experiments.