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Review
. 2002 Feb 1;22(3):619-23.
doi: 10.1523/JNEUROSCI.22-03-00619.2002.

Neurogenesis in adult mammals: some progress and problems

Elizabeth Gould  1 , Charles G Gross
Affiliations
Review

Neurogenesis in adult mammals: some progress and problems

Elizabeth Gould et al. J Neurosci. .
No abstract available

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Figures

Fig. 1.
Fig. 1.
Some new cells in the adult rodent and primate brain have neuronal characteristics. Top panel, Confocal image of a cell (arrow) in the anterior cortex of an adult rat double-labeled with NeuN (green nuclear and cytoplasmic stain; a marker for mature neurons) and BrdU (red nuclear stain; a marker of DNA synthesis). The image is rotated in orthogonal planes (x,y, z) to verify double labeling throughout its extent. The rat was perfused 3 weeks after the BrdU injection. Bottom panel, Confocal image of a cell (arrow) in prefrontal cortex of an adult macaque double-labeled with NeuN (green) and BrdU (red). The image is rotated in orthogonal planes (x, y, z) to verify double labeling throughout its extent. The monkey was perfused 2 weeks after the BrdU injection (adapted from Gould et al., 2001).
Fig. 2.
Fig. 2.
Methodological considerations related to BrdU labeling in adults. Top panel, A short pulse of BrdU labels in a dose-dependent manner, raising the possibility that changes in the number of BrdU-labeled cells could result from differences in BrdU availability. The graph shows the total number of BrdU-labeled cells in the dentate gyrus of adult rats after a single injection of BrdU 30 min earlier. *Indicates significant difference from all other groups, p < 0.05. **Indicates significant difference from 300 mg/kg group, p < 0.05. Adapted from Cameron and McKay (2001) with permission. Bottom panel,Left, BrdU-labeled progenitor cells in the subgranular zone (s) of the dentate gyrus. BrdU is an exogenous marker of proliferating cells.Right, Dividing cell undergoing cytokinesis labeled with phosphorylated histone H3 in the subgranular zone. p histone H3 is an endogenous marker of cells in M phase (Hendzel et al., 1997). Endogenous markers can be used to verify that changes in the number of BrdU-labeled cells are attributable to differences in the number of dividing cells, as opposed to difference in BrdU availability.g, Granule cell layer; h, hilus.
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