Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2002 Feb;5(2):98-103.
doi: 10.1006/mthe.2002.0524.

The nasal epithelium as a factory for systemic protein delivery

Uta Griesenbach  1 , Robin L Cassady, Stefano Ferrari, Masayuki Fukumura, Christian Müller, Edgar Schmitt, Jie Zhu, Peter K Jeffery, Yoshiyuki Nagai, Duncan M Geddes, Mamoru Hasegawa, Eric W F W Alton
Affiliations
Free article

The nasal epithelium as a factory for systemic protein delivery

Uta Griesenbach et al. Mol Ther. 2002 Feb.
Free article

Abstract

We have previously shown that recombinant Sendai virus (SeV) produces efficient in vivo airway epithelial gene transfer. The ability to produce therapeutic levels of circulating proteins following noninvasive gene transfer would have widespread clinical application. Here, we compared nose, lung, and skeletal muscle for the ability to produce circulating levels of the secreted mouse antiinflammatory cytokine interleukin-10 (IL10) following SeV-mediated gene transfer. High levels of serum IL10 were obtained from each site with a potency order of lung > nose > muscle for a given viral titer. Serum levels from each site were within the likely required range for anti-inflammatory effects. The combination of a high-efficiency gene transfer agent (SeV) and sites that can be assessed noninvasively (nose or lung) may circumvent several current challenges to gene therapy.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

  • Assessment of F/HN-pseudotyped lentivirus as a clinically relevant vector for lung gene therapy.
    Griesenbach U, Inoue M, Meng C, Farley R, Chan M, Newman NK, Brum A, You J, Kerton A, Shoemark A, Boyd AC, Davies JC, Higgins TE, Gill DR, Hyde SC, Innes JA, Porteous DJ, Hasegawa M, Alton EW. Griesenbach U, et al. Am J Respir Crit Care Med. 2012 Nov 1;186(9):846-56. doi: 10.1164/rccm.201206-1056OC. Epub 2012 Sep 6. Am J Respir Crit Care Med. 2012. PMID: 22955314 Free PMC article.
  • The murine lung as a factory to produce secreted intrapulmonary and circulatory proteins.
    Paul-Smith MC, Pytel KM, Gelinas JF, McIntosh J, Pringle I, Davies L, Chan M, Meng C, Bell R, Cammack L, Moran C, Cameron L, Inoue M, Tsugumine S, Hironaka T, Gill DR, Hyde SC, Nathwani A, Alton EWFW, Griesenbach U. Paul-Smith MC, et al. Gene Ther. 2018 Aug;25(5):345-358. doi: 10.1038/s41434-018-0025-8. Epub 2018 Jul 18. Gene Ther. 2018. PMID: 30022127 Free PMC article.
  • The use of carboxymethylcellulose gel to increase non-viral gene transfer in mouse airways.
    Griesenbach U, Meng C, Farley R, Wasowicz MY, Munkonge FM, Chan M, Stoneham C, Sumner-Jones SG, Pringle IA, Gill DR, Hyde SC, Stevenson B, Holder E, Ban H, Hasegawa M, Cheng SH, Scheule RK, Sinn PL, McCray PB Jr, Alton EW. Griesenbach U, et al. Biomaterials. 2010 Mar;31(9):2665-72. doi: 10.1016/j.biomaterials.2009.12.005. Epub 2009 Dec 21. Biomaterials. 2010. PMID: 20022367 Free PMC article.
  • Tetrafunctional Block Copolymers Promote Lung Gene Transfer in Newborn Piglets.
    Caballero I, Riou M, Hacquin O, Chevaleyre C, Barc C, Pezant J, Pinard A, Fassy J, Rezzonico R, Mari B, Heuzé-Vourc'h N, Pitard B, Vassaux G. Caballero I, et al. Mol Ther Nucleic Acids. 2019 Jun 7;16:186-193. doi: 10.1016/j.omtn.2019.02.016. Epub 2019 Feb 26. Mol Ther Nucleic Acids. 2019. PMID: 30897407 Free PMC article.
  • Correction of a chronic pulmonary disease through lentiviral vector-mediated protein expression.
    Lund-Palau H, Juarez-Molina CI, Meng C, Bhargava A, Pilou A, Aziz K, Clarke N, Atsumi N, Ashek A, Wilson MR, Takata M, Padley S, Gill DR, Hyde SC, Morgan C, Alton EWFW, Griesenbach U. Lund-Palau H, et al. Mol Ther Methods Clin Dev. 2022 Apr 14;25:382-391. doi: 10.1016/j.omtm.2022.04.002. eCollection 2022 Jun 9. Mol Ther Methods Clin Dev. 2022. PMID: 35573048 Free PMC article.

Publication types

LinkOut - more resources