[Effect of protein expression on antisense c-myc and PCNA for vascular smooth muscle cells]

Abstract

Objective: To study the methods of inhibiting proliferation of vascular smooth muscle cells (VSMCs), we observed the change of protein expression after using antisense c-myc, PCNA for VSMCs.

Methods: The sense, antisense, and mismatched oligomers for c-myc or PCNA were individually delivered to the cultured VSMCs. Using Western blot, immunohistochemistry assay and image analysis system, we observed the change of c-myc and PCNA protein expression in the VSMCs.

Results: Antisense c-myc, PCNA at concentration of 10 micromol/L can successfully inhibit the expression of c-myc, PCNA protein in the VSMCs (P < 0.05), but the sense and the mismatched do not have this effect (P > 0.05).

Conclusion: The data suggested that the antisense c-myc, PCNA could attenuate the protein expression of c-myc, PCNA, and it is an effective method for the inhibition of VSMCs hyperplasia.