[Effects of p16 and GM-CSF combined gene therapy on immune response of tumor-bearing mice]

Abstract

Objective: To investigate the effects of p16 and GM-CSF combined gene therapy on immune response of tumor-bearing mice (Renca).

Methods: The model of renal tumor-bearing mice was constructed by subcutaneous injection of Renca cells. The tumor-bearing mice were divided into 5 groups at random three days after injection of Renca cells. Different groups of mice were given different treatments. The antitumor immune responses and the survival time of mice were tested. Histopathologic analysis of tumor tissue was done.

Results: Enhancement of antitumor immune responses and prolongation of survival period were observed more obviously in tumor-bearing mice after combined therapy than in those of the other four groups.

Conclusions: p16 and GM-CSF combined gene therapy can enhance the antitumor immune responses of mice and inhibit the growth of pre-established renal cell carcinoma.