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. 2002 Feb;8(1):54-9.
doi: 10.3748/wjg.v8.i1.54.

Differential display of vincristine-resistance-related genes in gastric cancer SGC7901 cell

Xin Wang  1 , Mei Lan, Yong-Quan Shi, Ju Lu, Yue-Xia Zhong, Han-Ping Wu, Hui-Hong Zai, Jie Ding, Kai-Cun Wu, Bo-Rong Pan, Jian-Ping Jin, Dai-Ming Fan
Affiliations

Differential display of vincristine-resistance-related genes in gastric cancer SGC7901 cell

Xin Wang et al. World J Gastroenterol. 2002 Feb.

Abstract

Aim: To isolate and clone the vincristine-resistance-related genes in gastric cancer SGC7901 cell line and to clarify the multidrug-resistant molecular mechanism of gastric cancer cells.

Methods: The modified differential-display polymerase chain reaction (DD-PCR) was used to examine differences in the mRNA composition of Vincristine-resistant gastric cancer SGC 7901 cells (SGC7901/VCR), induced by vincristine sulfate versus SGC7901cells. The differentially expressed cDNA fragments were confirmed by reverse Northern analysis, sequencing, BLAST analysis and Northern bolt analysis.

Results: The DD-PCR identified that 54 cDNA fragments were preferentially expressed in SGC 7901/VCR cells. When these cDNA fragments were analyzed by reverse Northern blot, twenty were reproducibly expressed at high level in SGC7901/VCR. Sequencing and BLAST analysis revealed that seven of the genes were known genes:ADP-ribosylation factor 4, Cytochrome oxidase subunit II, Ss-A/Ro ribonucleoprtein autoantigen 60kd subunit,ribosomal protein S13, galaectin-8 gene, oligophrenin 1 mRNA, ribosomal protein L23 mRNA; thirteen of the genes were unknown genes. The length and abundance of the four unknown genes were further confirmed by Northern blot analysis.

Conclusion: The twenty differential known and unknown genes may be related to the vincristine-resistant mechanism in human gastric cancer SGC7901 cell line.

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Figures

Figure 1
Figure 1
Differential display of mRNA of SGC7901/VCR.
Figure 2
Figure 2
Restriction endonuclease cleavage identification of differential cDNA clones in SGC7901/VCR.
Figure 3
Figure 3
Reverse Northern analysis of genes significantly highly ex-pressed in SGC7901/VCR.
See this image and copyright information in PMC

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