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. 2002 Jan-Feb;34(1):38-42.
doi: 10.1159/000048323.

Gene expression of neurotrophins and their high-affinity Trk receptors in cultured human Müller cells

Hidehiro Oku  1 , Tsunehiko Ikeda, Youichi Honma, Chie Sotozono, Kohji Nishida, Yo Nakamura, Teruyo Kida, Shigeru Kinoshita
Affiliations

Gene expression of neurotrophins and their high-affinity Trk receptors in cultured human Müller cells

Hidehiro Oku et al. Ophthalmic Res. 2002 Jan-Feb.

Abstract

Purpose: To investigate the gene expression of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin 3 (NT-3) and their high-affinity receptors (TrkA, TrkB and TrkC) in cultured human Müller cells.

Methods: The polymerase chain reaction was performed using specific primers for NGF, BDNF, NT-3, TrkA, TrkB and TrkC with complementary DNAs as the template, which were generated from poly-A+ RNA of cultured human Müller cells.

Results: We detected the precursor mRNA transcripts for NGF, BDNF, NT-3, TrkB and TrkC, but not TrkA.

Conclusion: Human Müller cells can direct mRNA expressions of a number of neurotrophins which may have a neurotrophic function in the retina. An autocrine mode of action is suggested, since Müller cells not only synthesize neurotrophic factors, but also express their specific receptors.

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