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. 2002 Feb;13(2):166-9.
doi: 10.1016/S1044-0305(01)00348-8.

A study of peptide-peptide interactions using MALDI ion mobility o-TOF and ESI mass spectrometry

Amina S Woods  1 , John M Koomen, Brandon T Ruotolo, Kent J Gillig, David H Russel, Katrin Fuhrer, Marc Gonin, Thomas F Egan, J Albert Schultz
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Free article

A study of peptide-peptide interactions using MALDI ion mobility o-TOF and ESI mass spectrometry

Amina S Woods et al. J Am Soc Mass Spectrom. 2002 Feb.
Free article

Abstract

Matrix-assisted laser desorption ionization ion mobility coupled to orthogonal time-of-flight mass spectrometry (MALDI-IM-oTOF MS) is evaluated as a tool for studying non-covalent complex (NCX) formation between peptides. The NCX formed between dynorphin 1-7 and Mini Gastrin I is used as a model system for comparison to previous MALDI experiments (Woods, A. S.; Huestis, M. A. J. Am. Soc. Mass Spectrom. 2001, 12, 88-96). The dynorphin 1-7/Mini Gastrin I complex is stable after more than a ms drift time through the He filled mobility cell. Furthermore, the effects of solution pH on NCX ion signal intensity is measured both by MALDI-IM-MS analysis and by nanoelectrospray mass spectrometry. When compared to the previous MALDI study this work shows that all three techniques give similar results. In addition, fragmentation can be observed from of the non-covalent complex parent ion that occurs prior to TOF mass analysis but after mobility separation, thus providing NCX composition information.

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References

    1. J Am Chem Soc. 2001 Jun 20;123(24):5660-7 - PubMed
    1. Mass Spectrom Rev. 1997 Jan-Feb;16(1):1-23 - PubMed
    1. Anal Chem. 1999 Sep 15;71(18):3918-27 - PubMed
    1. J Am Chem Soc. 2001 Jun 13;123(23):5610-1 - PubMed
    1. Anal Chem. 1998 Dec 1;70(23):4929-38 - PubMed

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