Identification of microorganisms by gas chromatographic-mass spectrometric analysis of cellular fatty acids
- PMID: 1184690
- DOI: 10.1016/s0021-9673(00)99988-6
Identification of microorganisms by gas chromatographic-mass spectrometric analysis of cellular fatty acids
Abstract
The cellular fatty acid compositions of strains of Pseudomonas aeruginosa, P. cepacia, and P. maltophilia were determined by gas-liquid chromatography. A variety of acids was detected in these organisms, including branched and straight-chain acids, cyclopropane, and hydroxy acids. Comparison of the presence and relative amounts of these acids was useful in distinguishing the three species. Three branched-chain hydroxy acids not found in other bacteria were present in P. maltophilia and were identified by gas chromatography, mass spectrometry, and infrared spectroscopy.
Similar articles
-
Cellular fatty acid composition of selected Pseudomonas species.Appl Microbiol. 1972 Oct;24(4):596-8. doi: 10.1128/am.24.4.596-598.1972. Appl Microbiol. 1972. PMID: 4628797 Free PMC article.
-
Occurrence of branched-cahin hydroxy fatty acids in Pseudomonas maltophilia.J Bacteriol. 1973 Jun;114(3):1018-24. doi: 10.1128/jb.114.3.1018-1024.1973. J Bacteriol. 1973. PMID: 4712564 Free PMC article.
-
Cellular fatty acids of Alcaligenes and Pseudomonas species isolated from clinical specimens.J Clin Microbiol. 1975 May;1(5):414-9. doi: 10.1128/jcm.1.5.414-419.1975. J Clin Microbiol. 1975. PMID: 1176611 Free PMC article.
-
Development of gas chromatography analysis of fatty acids in marine organisms.J Chromatogr Sci. 2013 Aug;51(7):599-607. doi: 10.1093/chromsci/bmt005. Epub 2013 Jan 31. J Chromatogr Sci. 2013. PMID: 23377649 Review.
-
[THE CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF MICROBIAL FATTY ACIDS IN HUMAN BIOLOGICAL FLUIDS AND THEIR CLINICAL SIGNIFICANCE].Klin Lab Diagn. 2015 Dec;60(12):46-55. Klin Lab Diagn. 2015. PMID: 27032254 Review. Russian.
Cited by
-
Characterization of CDC group DF-3 by cellular fatty acid analysis.J Clin Microbiol. 1989 Apr;27(4):735-7. doi: 10.1128/jcm.27.4.735-737.1989. J Clin Microbiol. 1989. PMID: 2542365 Free PMC article.
-
Effect of Culture Conditions on Fatty Acid Profiles of Bacteria and Lipopolysaccharides of the Genus Pseudomonas-GC-MS Analysis on Ionic Liquid-Based Column.Molecules. 2022 Oct 15;27(20):6930. doi: 10.3390/molecules27206930. Molecules. 2022. PMID: 36296523 Free PMC article.
-
Description and genomic characterization of Cohnella caldifontis sp. nov., isolated from hot springs in Yunnan province, south-west China.Antonie Van Leeuwenhoek. 2024 Jan 8;117(1):20. doi: 10.1007/s10482-023-01908-y. Antonie Van Leeuwenhoek. 2024. PMID: 38189996
-
Identification of hydroxy fatty acids in Aeromonas hydrophila, Aeromonas sobria, and Aeromonas caviae.J Clin Microbiol. 1985 Dec;22(6):1061-2. doi: 10.1128/jcm.22.6.1061-1062.1985. J Clin Microbiol. 1985. PMID: 3934208 Free PMC article.
-
Location of double bonds in monounsaturated fatty acids of Campylobacter cryaerophila with dimethyl disulfide derivatives and combined gas chromatography-mass spectrometry.J Clin Microbiol. 1989 Jul;27(7):1467-70. doi: 10.1128/jcm.27.7.1467-1470.1989. J Clin Microbiol. 1989. PMID: 2768436 Free PMC article.