Method for extraction of glycoprotein hormones from plasma for use in radioimmunoassays
- PMID: 1185044
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Method for extraction of glycoprotein hormones from plasma for use in radioimmunoassays
J Lab Clin Med.
1975 Nov.
Abstract
Concanavalin-A covalently linked to Sepharose was used to extract the glycoproteins from a volume of human plasma about 10 times that volume usually added to conventional radioimmunoassay systems. Using a chorionic gonadoptropin (hCG) radioimmunoassay, hCG inhibition curves generated in the presence of the extracts were nearly identical to those generated by the conventional method; therefore, the sensitivity of the radioimmunoassays for glycoprotein hormones.
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