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. 2002 Feb 8;108(3):371-81.
doi: 10.1016/s0092-8674(02)00629-3.

A chemical-genetic strategy implicates myosin-1c in adaptation by hair cells

Jeffrey R Holt  1 , Susan K H Gillespie, D William Provance, Kavita Shah, Kevan M Shokat, David P Corey, John A Mercer, Peter G Gillespie
Affiliations
Free article

A chemical-genetic strategy implicates myosin-1c in adaptation by hair cells

Jeffrey R Holt et al. Cell. .
Free article

Abstract

Myosin-1c (also known as myosin-Ibeta) has been proposed to mediate the slow component of adaptation by hair cells, the sensory cells of the inner ear. To test this hypothesis, we mutated tyrosine-61 of myosin-1c to glycine, conferring susceptibility to inhibition by N(6)-modified ADP analogs. We expressed the mutant myosin-1c in utricular hair cells of transgenic mice, delivered an ADP analog through a whole-cell recording pipette, and found that the analog rapidly blocked adaptation to positive and negative deflections in transgenic cells but not in wild-type cells. The speed and specificity of inhibition suggests that myosin-1c participates in adaptation in hair cells.

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