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. 2001 Dec;7(6):783-7.
doi: 10.3748/wjg.v7.i6.783.

Polymorphism of flagellin A gene in Helicobacter pylori

W S Ji  1 , J L Hu, J W Qiu, D R Peng, B L Shi, S J Zhou, K C Wu, D M Fan
Affiliations

Polymorphism of flagellin A gene in Helicobacter pylori

W S Ji et al. World J Gastroenterol. 2001 Dec.

Abstract

Aim: To study the polymorphism of flagellin A genotype and its significance in Helicobacter pylori (H. pylori).

Methods: As the template, genome DNA was purified from six clinical isolates of H. pylori from outpatients, and the corresponding flagellin A fragments were amplified by polymerase chain reaction. All these products were sequenced. These sequences were compared with each other, and analyzed by software of FASTA program.

Results: Specific PCR products were amplified from all of these H. pylori isolates and no length divergence was found among them. Compared with each other, the highest ungapped identity is 99.10%, while the lowest is 94.65%. Using FASTA program, the alignments between query and library sequences derived from different H. pylori strains were higher than 90%.

Conclusion: The nucleotide sequence of flagellin A in H. pylori is highly conservative with incident divergence. This information may be useful for gene diagnosis and further study on flagellar antigen phenotype.

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Figures

Figure 1
Figure 1
Comparison of PCR products between sequence A and other sequences.
See this image and copyright information in PMC

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