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. 2001 Dec;7(6):783-7.
doi: 10.3748/wjg.v7.i6.783.

Polymorphism of flagellin A gene in Helicobacter pylori

W S Ji  1 , J L Hu, J W Qiu, D R Peng, B L Shi, S J Zhou, K C Wu, D M Fan
Affiliations

Polymorphism of flagellin A gene in Helicobacter pylori

W S Ji et al. World J Gastroenterol. 2001 Dec.

Abstract

Aim: To study the polymorphism of flagellin A genotype and its significance in Helicobacter pylori (H. pylori).

Methods: As the template, genome DNA was purified from six clinical isolates of H. pylori from outpatients, and the corresponding flagellin A fragments were amplified by polymerase chain reaction. All these products were sequenced. These sequences were compared with each other, and analyzed by software of FASTA program.

Results: Specific PCR products were amplified from all of these H. pylori isolates and no length divergence was found among them. Compared with each other, the highest ungapped identity is 99.10%, while the lowest is 94.65%. Using FASTA program, the alignments between query and library sequences derived from different H. pylori strains were higher than 90%.

Conclusion: The nucleotide sequence of flagellin A in H. pylori is highly conservative with incident divergence. This information may be useful for gene diagnosis and further study on flagellar antigen phenotype.

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Figures

Figure 1
Figure 1
Comparison of PCR products between sequence A and other sequences.
See this image and copyright information in PMC

References

    1. Kamiya S, Yamaguchi H, Osaki T, Taguchi H. A virulence factor of Helicobacter pylori: role of heat shock protein in mucosal inflammation after H. pylori infection. J Clin Gastroenterol. 1998;27 Suppl 1:S35–S39. - PubMed
    1. Domingo D, Alarcón T, Sanz JC, Villar H, Hernández JM, Sánchez J, López-Brea M. [The Helicobacter pylori adhesion gene: relation with the origin of the isolates and associated disease] Enferm Infecc Microbiol Clin. 1999;17:342–346. - PubMed
    1. Yokota Si, Amano Ki, Hayashi S, Kubota T, Fujii N, Yokochi T. Human antibody response to Helicobacter pylori lipopolysaccharide: presence of an immunodominant epitope in the polysaccharide chain of lipopolysaccharide. Infect Immun. 1998;66:3006–3011. - PMC - PubMed
    1. Monteiro MA, Chan KH, Rasko DA, Taylor DE, Zheng PY, Appelmelk BJ, Wirth HP, Yang M, Blaser MJ, Hynes SO, et al. Simultaneous expression of type 1 and type 2 Lewis blood group antigens by Helicobacter pylori lipopolysaccharides. Molecular mimicry between H. pylori lipopolysaccharides and human gastric epithelial cell surface glycoforms. J Biol Chem. 1998;273:11533–11543. - PubMed
    1. Yamaguchi H, Osaki T, Taguchi H, Hanawa T, Yamamoto T, Fukuda M, Kawakami H, Hirano H, Kamiya S. Growth inhibition of Helicobacter pylori by monoclonal antibody to heat-shock protein 60. Microbiol Immunol. 1997;41:909–916. - PubMed

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