Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2001 Dec;7(6):836-40.
doi: 10.3748/wjg.v7.i6.836.

Expression of lipopolysaccharide binding protein and its receptor CD14 in experimental alcoholic liver disease

G Q Zuo  1 , J P Gong, C A Liu, S W Li, X C Wu, K Yang, Y Li
Affiliations

Expression of lipopolysaccharide binding protein and its receptor CD14 in experimental alcoholic liver disease

G Q Zuo et al. World J Gastroenterol. 2001 Dec.

Abstract

Aim: To evaluate the relationship between the expression of lipopolysaccharides (LPS) binding protein (LBP) and CD14 mRNA and the severity of liver injury in alcohol-fed rats.

Methods: Twenty Wistar rats were divided into two groups:ethanol-fed group (group E) and control group (group C). Group E was fed with ethanol(5-12 g x kg(-1) x d(-1)) and group C received dextrose instead of ethanol. Rats of the two groups were sacrificed at 4 weeks and 8 weeks. Levels of endotoxin and alanine transaminase (ALT) in blood were measured, and liver pathology was observed under light and electronic microscopy. Expressions of LBP and CD14 mRNA in liver tissues were determined by RT-PCR analysis.

Results: Plasma endotoxin levels were increased more significantly in group E(129+/-21) ng x L(-1) and (187+/-35) ng x L(-1) at 4 and 8 wk than in control rats(48+/-9) ng x L(-1) and (53+/-11) ng x L(-1), respectively (P<0.05). Mean values of plasma ALT levels were (1867+/-250) nkat x L(-1) and (2450+/-367) nkat x L(-1) in Group E. The values were increased more dramatically in ethanol-fed rats than in Group C after 4 and 8 weeks. In liver section from ethanol-fed rats, there were marked pathological changes (steatosis, cell infiltration and necrosis). In ethanol-fed rats, ethanol administration led to a significant increase in LBP and CD14 mRNA levels compared with the control group (P<0.05).

Conclusion: Ethanol administration led to a significant increase in endotoxin levels in serum and LBP and CD14 mRNA expressions in liver tissues. The increase of LBP and CD14 mRNA expression might wake the liver more sensitive to endotoxin and liver injury.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Chenges of endotoxin levels in two groups at different time points.
Figure 2
Figure 2
Changes of serum transaminase levels in two groups at different time points.
Figure 3
Figure 3
Liver section from rats (HE × 200) A: 4wk after ethanol liquid diet, steatosis and accumulation of blood cells in the sinusoidal lining; B: 8wk after ethanol diet, steatosis, cell infiltration and necrosis.
Figure 4
Figure 4
Ethanol-fed rats. A: Steatosis degeneration and necrosis in hepatocytes TEM × 5000 B: Focal cytoplasmic degeneration and many myelin figures (TEM × 20000).
Figure 5
Figure 5
Expression of LBP and CD14 mRNA by RT-PCR analysis. M: Marker; Lane 1, 3: Group C in 4, 8 wk respectively. Lane 2, 4: Group E in 4, 8 wk.
Figure 6
Figure 6
Expression of LBP mRNA.
Figure 7
Figure 7
Expression of CD14 mRNA.
See this image and copyright information in PMC

References

    1. Fujimoto M, Uemura M, Nakatani Y, Tsujita S, Hoppo K, Tamagawa T, Kitano H, Kikukawa M, Ann T, Ishii Y, et al. Plasma endotoxin and serum cytokine levels in patients with alcoholic hepatitis: relation to severity of liver disturbance. Alcohol Clin Exp Res. 2000;24:48S–54S. - PubMed
    1. Zhang SC, Dai Q, Wang JY, He BM, Zhou K. Gut derived endotoxemia: one of the factors leading to production of cytokines in liver diseases. World J Gastroenterol. 2000;6(Suppl 3):16.
    1. Gordon H. Detection of alcoholic liver disease. World J Gastroenterol. 2001;7:297–302. - PMC - PubMed
    1. Zhang GL, Wang YH, Teng HL, Lin ZB. Effects of aminoguanidine on nitric oxide production induced by inflammatory cytokines and endotoxin in cultured rat hepatocytes. World J Gastroenterol. 2001;7:331–334. - PMC - PubMed
    1. Enomoto N, Yamashina S, Kono H, Schemmer P, Rivera CA, Enomoto A, Nishiura T, Nishimura T, Brenner DA, Thurman RG. Development of a new, simple rat model of early alcohol-induced liver injury based on sensitization of Kupffer cells. Hepatology. 1999;29:1680–1689. - PubMed

Publication types