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. 2001 Dec;7(6):836-40.
doi: 10.3748/wjg.v7.i6.836.

Expression of lipopolysaccharide binding protein and its receptor CD14 in experimental alcoholic liver disease

G Q Zuo  1 , J P Gong, C A Liu, S W Li, X C Wu, K Yang, Y Li
Affiliations

Expression of lipopolysaccharide binding protein and its receptor CD14 in experimental alcoholic liver disease

G Q Zuo et al. World J Gastroenterol. 2001 Dec.

Abstract

Aim: To evaluate the relationship between the expression of lipopolysaccharides (LPS) binding protein (LBP) and CD14 mRNA and the severity of liver injury in alcohol-fed rats.

Methods: Twenty Wistar rats were divided into two groups:ethanol-fed group (group E) and control group (group C). Group E was fed with ethanol(5-12 g x kg(-1) x d(-1)) and group C received dextrose instead of ethanol. Rats of the two groups were sacrificed at 4 weeks and 8 weeks. Levels of endotoxin and alanine transaminase (ALT) in blood were measured, and liver pathology was observed under light and electronic microscopy. Expressions of LBP and CD14 mRNA in liver tissues were determined by RT-PCR analysis.

Results: Plasma endotoxin levels were increased more significantly in group E(129+/-21) ng x L(-1) and (187+/-35) ng x L(-1) at 4 and 8 wk than in control rats(48+/-9) ng x L(-1) and (53+/-11) ng x L(-1), respectively (P<0.05). Mean values of plasma ALT levels were (1867+/-250) nkat x L(-1) and (2450+/-367) nkat x L(-1) in Group E. The values were increased more dramatically in ethanol-fed rats than in Group C after 4 and 8 weeks. In liver section from ethanol-fed rats, there were marked pathological changes (steatosis, cell infiltration and necrosis). In ethanol-fed rats, ethanol administration led to a significant increase in LBP and CD14 mRNA levels compared with the control group (P<0.05).

Conclusion: Ethanol administration led to a significant increase in endotoxin levels in serum and LBP and CD14 mRNA expressions in liver tissues. The increase of LBP and CD14 mRNA expression might wake the liver more sensitive to endotoxin and liver injury.

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Figures

Figure 1
Figure 1
Chenges of endotoxin levels in two groups at different time points.
Figure 2
Figure 2
Changes of serum transaminase levels in two groups at different time points.
Figure 3
Figure 3
Liver section from rats (HE × 200) A: 4wk after ethanol liquid diet, steatosis and accumulation of blood cells in the sinusoidal lining; B: 8wk after ethanol diet, steatosis, cell infiltration and necrosis.
Figure 4
Figure 4
Ethanol-fed rats. A: Steatosis degeneration and necrosis in hepatocytes TEM × 5000 B: Focal cytoplasmic degeneration and many myelin figures (TEM × 20000).
Figure 5
Figure 5
Expression of LBP and CD14 mRNA by RT-PCR analysis. M: Marker; Lane 1, 3: Group C in 4, 8 wk respectively. Lane 2, 4: Group E in 4, 8 wk.
Figure 6
Figure 6
Expression of LBP mRNA.
Figure 7
Figure 7
Expression of CD14 mRNA.
See this image and copyright information in PMC

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