Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2002 Feb;93(2):198-208.
doi: 10.1111/j.1349-7006.2002.tb01259.x.

Serine proteinase inhibitor 9 can be recognized by cytotoxic T lymphocytes of epithelial cancer patients

Koji Tanaka  1 , Nanae Harashima, Fumihiko Niiya, Yoshiaki Miyagi, Naoya Hida, Mika Ochi, Nobue Imai, Mamoru Harada, Kyogo Itoh, Shigeki Shichijo
Affiliations

Serine proteinase inhibitor 9 can be recognized by cytotoxic T lymphocytes of epithelial cancer patients

Koji Tanaka et al. Jpn J Cancer Res. 2002 Feb.

Abstract

Serine proteinase inhibitor 9 (PI-9) inhibits granzyme B-mediated apoptosis and interleukin-1beta-converting enzyme activity. In this study, we report that the PI-9 gene encodes antigenic epitopes recognized by the HLA-A24-restricted and tumor-reactive cytotoxic T lymphocytes (CTLs) of epithelial cancer patients. Screening of an autologous cDNA library using a CTL line recognizing HLA-A24+ tumor cells resulted in the isolation of a cDNA, which had an identical coding region to the previously described PI-9 genes. PI-9 gene was expressed in approximately three-fourths of epithelial cancer cell lines and all leukemic cell lines tested. It was also expressed in normal peripheral blood mononuclear cells (PBMCs), but not in a normal fibroblast cell line. CTL sublines contained T cells capable of recognizing the PI-9(292-300) and PI-9(348-356) peptides among 13 different peptides having the HLA-A24 binding motifs. These two peptides were recognized by the CTL line in a dose-dependent and HLA class-I-restricted manner, and also possessed the ability to induce HLA class I-restricted and tumor-reactive CTLs in PBMCs from HLA-A24+ cancer patients. These results demonstrate that PI-9 is recognized by HLA class I-restricted and tumor-reactive CTLs of epithelial cancer patients.

PubMed Disclaimer

References

    1. van der Bruggen , P. , Traversari , C. , Chomez , P. , Lurqin , C. , Plaen , E. D. , van der Eynde , B. , Knuth , A. and Boon , T.A gene encoding an antigen recognized by cytotoxic T lymphocytes on a human melanoma . Science , 254 , 1643 – 1647 ( 1991. ). - PubMed
    1. Kawakami , Y. , Eliyahu , S. , Sakaguchi , K. , Robbins , P. F. , Rivoltini , L. , Yannelli , J. B. , Appella , E. and Rosenberg , S. A.Identification of the immunodominant peptides of the MART–1 human melanoma antigen recognized by the majority of HLA‐A2 restricted tumor infiltrating lymphocytes . J. Exp. Med. , 180 , 347 – 352 ( 1994. ). - PMC - PubMed
    1. Kawakami , Y. , Eliyahu , S. , Delgado , C. H. , Robbins , P. F. , Sakaguchi , K. , Appella , E. and Rosenberg , S. A.Identification of a human melanoma antigen recognized by tumor‐ infiltrating lymphocytes associated with in vivo tumor rejection . Proc. Natl. Acad. Set. USA , 91 , 6465 – 6462 ( 1994. ). - PMC - PubMed
    1. Brichard , V. , van Pel , A. , Wolfel , T. , de Plaen , E. , Lethe , B. , Coulie , P. and Boon , T.The tyrosinase gene codes for an antigen recognized by autologous cytolytic T lymphocytes on HLA‐A2 melanomas . J. Exp. Med. , 178 , 489 – 495 ( 1995. ). - PMC - PubMed
    1. Robbins , P. F. , EI‐Gamil , M. , Li , Y. F. , Kawakami , Y. , Loftus , D. , Appella , E. and Rosenberg , S. A.A mutated β‐catenin gene encodes a melanoma‐specific antigen recognized by tumor infiltrating lymphocytes . J. Exp. Med. , 183 , 1185 – 1192 ( 1996. ). - PMC - PubMed

Publication types

Associated data

LinkOut - more resources