Video scanning for the quantitative determination of alkaline phosphatase activity in the rat kidney

Abstract

The use of a video scanning equipment (Opton Micro-Videomat) for the quantitative determination of alkaline phosphatase activity in histological sections of the rat kidneys is presented. A photomicroscope with a telecamera, a video monitor, a computer and a recorder are incorporated into the system. Automatic measurement, accelerated detection, accuracy, sensitivity and reproducibility, as well as elimination of time-consuming work involved in other available methods are found to be the main advantage of the method.