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. 2002 Mar;68(3):1109-14.
doi: 10.1128/AEM.68.3.1109-1114.2002.

Quantitative trait loci controlling vegetative growth rate in the edible basidiomycete Pleurotus ostreatus

Luis M Larraya  1 , Eneko Idareta, Dani Arana, Enrique Ritter, Antonio G Pisabarro, Lucia Ramírez
Affiliations

Quantitative trait loci controlling vegetative growth rate in the edible basidiomycete Pleurotus ostreatus

Luis M Larraya et al. Appl Environ Microbiol. 2002 Mar.

Abstract

Mycelium growth rate is a quantitative characteristic that exhibits continuous variation. This trait has applied interest, as growth rate is correlated with production yield and increased advantage against competitors. In this work, we studied growth rate variation in the edible basidiomycete Pleurotus ostreatus growing as monokaryotic or dikaryotic mycelium on Eger medium or on wheat straw. Our analysis resulted in identification of several genomic regions (quantitative trait loci [QTLs]) involved in the control of growth rate that can be mapped on the genetic linkage map of this fungus. In some cases monokaryotic and dikaryotic QTLs clustered at the same map position, indicating that there are principal genomic areas responsible for growth rate control. The availability of this linkage map of growth rate QTLs can help in the design of rational strain breeding programs based on genomic information.

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Figures

FIG. 1.
FIG. 1.
Linkage map of P. ostreatus (21) and locations of QTL and digenic interactions that affect vegetative growth rate in monokaryons and dikaryons. The approximate positions of QTLs are indicated to the right of the markers. The percentages of the phenotypic variation explained by the putative QTLs (R2) are represented by proportional boxes. Significant interactions among QTLs, genes, and other molecular markers are indicated by arrows and are numbered as shown in Table 3. Randomly amplified polymorphic DNA markers on the map are indicated by the primer name and the approximate length of the corresponding amplification product. Randomly amplified polymorphic markers converted to restriction fragment length polymorphism markers are underlined. Genes are in italics. Markers that deviated from the expected 1:1 segregation (P < 0.05) are indicated by asterisks.
FIG. 2.
FIG. 2.
Continuous variation of the monokaryotic growth rate in straw-based substrate of the total monokaryotic population (⧫) and of the two populations differing in the est1 allele, est1-1 (▴) and est1-2 (▪).
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References

    1. Ballero, M., E. Mascia, A. Rescigno, and E. S. D. Teulada. 1990. Use of Pleurotus for transformation of polyphenols in waste waters from olive presses into proteins. Micol. Ital. 19:39-41.
    1. Beyer, D. M., P. J. Wuest, and J. J. Kremser. 2000. Evaluation of epidemiological factors and mushroom substrate characteristics influencing the occurrence and development of Trichoderma green mold, p. 633-640. In L. J. L. D. van Griensven (ed.), Science and cultivation of edible fungi, vol. 2. A. A. Balkema, Rotterdam, The Netherlands.
    1. Bos, I., and P. Caligari. 1995. Selection methods in plant breeding, 1st ed. Chapman & Hall, London, United Kingdom.
    1. Chang, R. 1996. Functional properties of edible mushrooms. Nutr. Rev. 54:91-93. - PubMed
    1. Chang, Y. C., L. A. Penoyer, and K. J. Kwon-Chung. 1996. The second capsule gene of Cryptococcus neoformans, CAP64, is essential for virulence. Infect. Immun. 64:1977-1983. - PMC - PubMed

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