Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 Oct;2(10):1745-9.
doi: 10.1093/nar/2.10.1745.

Reaction of a nucleoside 2, 4-dinitrophenyl phosphate with fluoride; a convenient method for the preparation of the nucleoside phosphorfluoridate

P W Johnson, R von Tigerstrom, M Smith
Free PMC article

Reaction of a nucleoside 2, 4-dinitrophenyl phosphate with fluoride; a convenient method for the preparation of the nucleoside phosphorfluoridate

P W Johnson et al. Nucleic Acids Res. 1975 Oct.
Free PMC article

Abstract

Examination of the reaction of 2, 4-dinitrofluorobenzene with thymidine-5' phosphate in detail reveals that the initial product is the 2, 4-dinitrophenyl ester. This reacts with fluoride to produce thymidine-5' phosphorfluoridate. This second reaction provides the basis for the conversion of preformed thymidine-5' 2, 4-dinitrophenyl phosphate to thymidine-5' phosphorofluoridate.

PubMed Disclaimer

References

    1. Biochemistry. 1969 Jul;8(7):3067-70 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1436-40 - PubMed
    1. Science. 1970 Feb 27;167(3922):1266-8 - PubMed
    1. Science. 1969 Jun 20;164(3886):1408-10 - PubMed
    1. J Org Chem. 1966 Oct;31(10):3241-6 - PubMed