Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2001 Sep;3(3):133-41.
doi: 10.1053/jfms.2001.0132.

Detection of feline leukaemia virus in blood and bone marrow of cats with varying suspicion of latent infection

E S Herring  1 , G C Troy, T E Toth, S D Forrester, L A Weigt, I P Herring
Affiliations

Detection of feline leukaemia virus in blood and bone marrow of cats with varying suspicion of latent infection

E S Herring et al. J Feline Med Surg. 2001 Sep.

Abstract

The purpose of this study was to determine if polymerase chain reaction (PCR) could be used to detect FeLV proviral DNA in bone marrow samples of cats with varying suspicion of latent infection. Blood and bone marrow samples from 50 cats and bone marrow from one fetus were collected, including 16 cats with diseases suspected to be FeLV-associated. Serum enzyme-linked immunosorbent assay (ELISA), blood and bone marrow immunofluorescent antibody test (IFA), and blood and bone marrow PCR were performed on each cat, and IFA and PCR on bone marrow of the fetus. Forty-one cats were FeLV negative. Five cats and one fetus were persistently infected with FeLV. Four cats had discordant test results. No cats were positive on bone marrow PCR only. It appears persistent or latent FeLV infection is not always present in conditions classically associated with FeLV.

PubMed Disclaimer

Figures

Fig 1.
Fig 1.
Results of polymerase chain reaction of feline leukaemia virus on ethidium-bromide stained gel. Expected size 495 bp. Lane 1, 100 bp molecular size marker, arrow indicates 500 bp; 2, PCR product from FL74 (persistently infected FeLV cell culture) used as positive control; 3, PCR product from negative control; 4, PCR product from blood of cat 12; 5, PCR product from bone marrow of cat 12; 6, PCR product from blood of cat 10; 7, PCR product from bone marrow of cat 35.
See this image and copyright information in PMC

References

    1. Altschul S, Madden T, Schaffer A, Zhang J, Zhang Z, Miller W, Lipman DJ. (1997) Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acid Research 25, 3389–3402. - PMC - PubMed
    1. Borchers K, Wolfinger U, Ludwig H. (1999) Latency-associated transcripts of equine herpesvirus type 4 in trigeminal ganglia of naturally infected horses. Journal of General Virology 80, 2165–2171. - PubMed
    1. Casey JW, Roach A, Mullins JI, Burck KB, Nicolson MO, Gardner MB, Davidson N. (1981) The U3 portion of feline leukaemia virus DNA identifies horizontally acquired proviruses in leukemic cats. Proceedings of National Academy of Science USA 7778–7782. - PMC - PubMed
    1. Cotter S. (1998) Feline viral neoplasia. In: Infectious Diseases of the Dog and Cat. Greene C. (ed). Philadelphia, WB Saunders, pp. 71–84.
    1. Egyed L, Bartha A. (1998) PCR studies on the potential sites for latency of BHV-4 in calves. Veterinary Research Communications 22, 209–216. - PubMed

Publication types

MeSH terms

LinkOut - more resources