The incorporation of radioactive uridine into the hepatitis B antigen of a chimpanzee
- PMID: 1187682
- DOI: 10.3181/00379727-150-38986
The incorporation of radioactive uridine into the hepatitis B antigen of a chimpanzee
Abstract
Radioactive (3H) uridine was incorporated into RNA isolated from the HBsAg of a chimpanzee carrier. HBsAg was purified by precipitation as an immune complex with the IgG fraction of chimpanzee anti-HBs. RNA was extracted from the washed complex with buffered phenol precipitated with alcohol and four nucleotides were identified by thin layer chromatography after alkali degradation.
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