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. 1975 Oct;150(1):121-5.
doi: 10.3181/00379727-150-38986.

The incorporation of radioactive uridine into the hepatitis B antigen of a chimpanzee

The incorporation of radioactive uridine into the hepatitis B antigen of a chimpanzee

W Jóźwiak et al. Proc Soc Exp Biol Med. 1975 Oct.

Abstract

Radioactive (3H) uridine was incorporated into RNA isolated from the HBsAg of a chimpanzee carrier. HBsAg was purified by precipitation as an immune complex with the IgG fraction of chimpanzee anti-HBs. RNA was extracted from the washed complex with buffered phenol precipitated with alcohol and four nucleotides were identified by thin layer chromatography after alkali degradation.

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