Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2002 Apr;102(1-2):67-71.
doi: 10.1016/s0166-0934(01)00450-5.

Prevalence of canine coronavirus antibodies by an enzyme-linked immunosorbent assay in dogs in the south of Italy

Annamaria Pratelli  1 , Gabriella Elia, Vito Martella, Alessandra Palmieri, Francesco Cirone, Antonella Tinelli, Marialaura Corrente, Canio Buonavoglia
Affiliations

Prevalence of canine coronavirus antibodies by an enzyme-linked immunosorbent assay in dogs in the south of Italy

Annamaria Pratelli et al. J Virol Methods. 2002 Apr.

Abstract

An enzyme-linked immunosorbent assay (Elisa), using as antigen canine coronavirus-infected CrFK cell supernatant, was developed to detect antibodies against canine coronavirus (CCoV). Out of a total of 109 dog serum samples, 80 which were positive by routine virus neutralisation test were also Elisa positive. Seventeen samples which were negative by the virus neutralisation test, were positive by Elisa and by the confirmatory Western blotting test. The Elisa was substantially more sensitive than the virus neutralisation test in detecting antibodies to CCoV and may be used as an alternative technique to virus neutralisation.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Evaluation of antibodies to CCoV in dog serum samples, using Elisa compared to virus neutralisation alone (A) and in combination with Western blotting analysis (B). Values in the boxes are numbers of samples.
See this image and copyright information in PMC

References

    1. Athanssious R., Marsolais G., Assaf R., Dea S., Descoteaux J.P., Dulude S., Montpetit C. Detection of bovine coronavirus and type A rotavirus in neonatal calf diarrhea and winter dyssentery of cattle in Quebec: evaluation of three diagnostic methods. Can. Vet. J. 1994;35:163–169. - PMC - PubMed
    1. Bandai C., Ishiguro S., Masuya N., Hohdatsu T., Mochizuki M. Canine coronavirus infections in Japan: virological and epidemiological aspects. J. Vet. Med. Sci. 1999;61:731–736. - PubMed
    1. Binn L.N., Lazar E.C., Keenan K.P., Huxsoll D.L., Marchwicki R.H., Strano A.J. Recovery and characterization of a coronavirus from military dogs with diarrhea. Proc. Annu. Mtg. US Anim. Health Assoc. 1974;78:359–366. - PubMed
    1. Buonavoglia, C., Marsilio, F., Cavalli, A., Tiscar, P.G., 1994. L'infezione da coronavirus del cane: indagine sulla presenza del virus in Italia, Not. Farm. Vet. Nr. 2/94, ed. SCIVAC.
    1. Carmichael L.E., Binn L.N. New canine enteric viral infection. Adv. Vet. Sci. 1981;25:1–37. - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources