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. 2002 Mar;40(3):794-8.
doi: 10.1128/JCM.40.3.794-798.2002.

Food-borne outbreak of gastroenteritis associated with genogroup I calicivirus

P J Hugo Johansson  1 , Maria Torvén, Ann-Christin Hammarlund, Ulla Björne, Kjell-Olof Hedlund, Lennart Svensson
Affiliations

Food-borne outbreak of gastroenteritis associated with genogroup I calicivirus

P J Hugo Johansson et al. J Clin Microbiol. 2002 Mar.

Abstract

An outbreak of gastroenteritis affecting 158 of 219 (72%) guests and employees at a hotel is described. Food served at the hotel restaurant is believed to have been the source of the outbreak and to have been contaminated by sick employees working in the restaurant. A secondary attack rate of 22% was seen involving 43 persons in all. In stool specimens from seven of eight patients, Norwalk-like viruses (NLVs) were detected by electron microscopy. While NLV-specific PCR using primers JV12 and JV13 were negative, all specimens examined with primers NVp69 and NVp110 were positive. The failure of primers JV12 and JV13 was attributed to several mismatches in the JV12 primer. Genotyping and sequence analysis revealed that all samples had identical sequences and clustered with genogroup I, and the most closely related well-characterized genotype is Desert Shield. This is the first described food-borne outbreak associated with genogroup I virus in Sweden.

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Figures

FIG. 1.
FIG. 1.
Onset of symptoms for 147 patients with gastroenteritis from 2 to 10 May 2000.
FIG. 2.
FIG. 2.
Age distribution of 147 patients with gastroenteritis.
FIG. 3.
FIG. 3.
Dendrogram based on a 321-nt stretch in the polymerase gene showing phylogenetic relationships between the identical samples from this outbreak and some prototype strains of calicivirus obtained from GenBank (accession numbers: SOV, Southhampton L07418; NV, Norwalk M87661; DSV, Desert Shield U04538; MX, Mexico U22498; LDV, Lordsdale X86557; MV, Melksham X81879). The numbers on the branches show the predicted nucleotide differences.
See this image and copyright information in PMC

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