Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2002 Mar;40(3):926-32.
doi: 10.1128/JCM.40.3.926-932.2002.

Detection of neutralizing antibodies against human papillomaviruses (HPV) by inhibition of gene transfer mediated by HPV pseudovirions

Latifa Bousarghin  1 , Alba-Lucia Combita-Rojas, Antoine Touzé, Slimane El Mehdaoui, Pierre-Yves Sizaret, Maria-Mercedes Bravo, Pierre Coursaget
Affiliations

Detection of neutralizing antibodies against human papillomaviruses (HPV) by inhibition of gene transfer mediated by HPV pseudovirions

Latifa Bousarghin et al. J Clin Microbiol. 2002 Mar.

Abstract

The goal of this study was to develop a human papillomavirus (HPV) neutralization assay using HPV pseudovirions generated in vitro. For this purpose, gene transfer efficiency of HPV virus-like particles (VLPs) was improved by using direct interaction between a reporter plasmid and the VLPs. Electron microscopic observation of the interaction between DNA molecules and VLPs revealed that VLPs always interact with a single DNA molecule and that VLPs bind to the end of linearized DNA molecules. An 100-fold improvement in the gene transfer was obtained by simple interaction between a linearized DNA molecule and VLPs. Moreover, direct interaction methods offer the possibility of transferring plasmids a size higher than that of the papillomavirus genome. The approach that we developed to generate HPV-16 and HPV-31 pseudovirions proved to be suitable for testing neutralizing antibodies in human sera both after immunization and after natural infection.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Electron micrographs of interactions observed between HPV-31 VLPs and heterologous DNA under circular (a and b) and linear forms (d and e) Bar, 100 nm. Bottom row, control DNA circular (c) and linear (f) molecules.
See this image and copyright information in PMC

References

    1. Barr, S. M., K. Keck, and H. V. Aposhian. 1979. Cell-free assembly of a polyoma-like particle from empty capsids and DNA. Virology 96:656-659. - PubMed
    1. Bonnez, W., R. C. Rose, C. Da Rin, C. Borkhuis, K. L. de Mesy Jensen, and R. C. Reichman. 1993. Propagation of human papillomavirus type 11 in human xenografts using the severe combined immunodeficiency (SCID) mouse and comparison to the nude mouse model. Virology 197:455-458. - PubMed
    1. Brady, J. N., V. D. Winston, and R. A. Consigli. 1977. Dissociation of polyomavirus by chelation of calcium ions found associated with purified virions. J. Virol. 23:717-724. - PMC - PubMed
    1. Carter, J. J., L. A. Koutsky, J. P. Hughes, S. K. Lee, J. Kuypers, N. Kiviat, and D. A. Galloway. 2000. Comparison of human papillomavirus type 16, 18, and 6 capsid antibody responses following incident infection. J. Infect. Dis. 181:1911-1919. - PubMed
    1. Christensen, N. D., J. W. Kreider, K. V. Shah, and R. F. Rande. 1992. Detection of human serum antibodies that neutralize infectious human papillomavirus type 11 virions. J. Gen. Virol. 73:1261-1267. - PubMed

Publication types

LinkOut - more resources