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. 2002 Mar;40(3):1088-90.
doi: 10.1128/JCM.40.3.1088-1090.2002.

Results of human papillomavirus DNA testing with the hybrid capture 2 assay are reproducible

Philip E Castle  1 , Attila T Lorincz, Iwona Mielzynska-Lohnas, David R Scott, Andrew G Glass, Mark E Sherman, John E Schussler, Mark Schiffman
Affiliations

Results of human papillomavirus DNA testing with the hybrid capture 2 assay are reproducible

Philip E Castle et al. J Clin Microbiol. 2002 Mar.

Abstract

Reproducibility of the Hybrid Capture 2 Test (HC 2) for human papillomavirus (HPV) DNA detection was evaluated by assaying frozen cervical specimens in 1997 and again in 2001 from 1,775 women with normal cervical cytology. Using a cutoff point of 1.0 pg of HPV DNA/ml between a negative and a positive test result, the result of the kappa test for agreement was 0.72 (a kappa value of >0.60 is considered good agreement). Using cutoff points of 1.0 and 10.0 pg/ml between negative and low positive and between low positive and high positive, respectively, the kappa was 0.68 and the linear-weighted kappa was 0.76. The results of this study indicate that HC 2 testing is reproducible even among cytologically normal women with low test values.

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Figures

FIG. 1.
FIG. 1.
Plot of test 1 (1997) results versus test 2 (2001) results.
See this image and copyright information in PMC

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