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. 2002 Feb;40(2):101-6.
doi: 10.1093/chromsci/40.2.101.

Evaluation of select variables in the ion chromatographic determination of F-, Cl-, Br-, NO(-)3, SO(-2)4, and PO(-3)4 in serum samples

Z Benzo  1 , A Escalona, J Salas, C Gómez, M Quintal, E Marcano, F Ruiz, A Garaboto, F Bartoli
Affiliations

Evaluation of select variables in the ion chromatographic determination of F-, Cl-, Br-, NO(-)3, SO(-2)4, and PO(-3)4 in serum samples

Z Benzo et al. J Chromatogr Sci. 2002 Feb.

Abstract

A full experimental design at two levels is applied for the estimation of the significance of select factors that may influence the ion chromatography (IC) determination of F-, Cl-, Br-, NO(-)3, SO(-2)4, and PO(-3)4 in serum samples. The factors studied are various sample deproteinization procedures, eluent composition, and flow rates. Deproteinization using either acetonitrile-NaOH or ultrafiltration can be used in order to obtain a significant protein removal before IC analysis; however, the former is recommended because it is less time-consuming and cheaper. Better resolution is obtained when a sodium hydroxide solution is used as the eluent. There is no influence of the sample's deproteinization procedures on the chromatographic resolution.

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