doi: 10.1126/science.1188358.
Guanidinium-CsCl density gradients for isopycnic analysis of nucleic acids
- PMID: 1188358
- DOI: 10.1126/science.1188358
Item in Clipboard
Guanidinium-CsCl density gradients for isopycnic analysis of nucleic acids
Science.
.
Abstract
The addition of guanidinium chloride to CsCl gradients lowers the apparent density of RNA, DNA, and hybrid polymers in such a way that all three can be banded, fractionated, and analyzed in one gradient with essentially no damage to their chemical integrity or to their biological activity.
Similar articles
-
Isopycnic-centrifugation studies in caesium chloride and in caesium sulphate on dermatan sulphate proteoglycans from bovine sclera.Biochem J. 1981 Dec 1;199(3):581-9. doi: 10.1042/bj1990581. Biochem J. 1981. PMID: 7340820 Free PMC article.
-
Advantages of rubidium chloride gradients for isopycnic centrifugation of enzymes.Biochem Int. 1984 Apr;8(4):471-6. Biochem Int. 1984. PMID: 6206870
-
Isolation of bacterial plasmids by density gradient centrifugation in cesium trifluoroacetate (CsTFA) without the use of ethidium bromide.Plasmid. 1985 Jan;13(1):78-80. doi: 10.1016/0147-619x(85)90059-9. Plasmid. 1985. PMID: 3887442
-
Modified method for RNA isolation from bacteria by guanidinium thiocyanate-cesium chloride centrifugation.Biotechniques. 1995 Apr;18(4):570-2. Biotechniques. 1995. PMID: 7541212 No abstract available.
-
Ultrastructural methods for nucleic acid detection by immunocytology.Prog Histochem Cytochem. 1999;34(2):87-159. doi: 10.1016/s0079-6336(99)80008-4. Prog Histochem Cytochem. 1999. PMID: 10546283 Review.
Cited by
-
cDNA cloning of a serotonin 5-HT1C receptor by electrophysiological assays of mRNA-injected Xenopus oocytes.Proc Natl Acad Sci U S A. 1987 Jun;84(12):4332-6. doi: 10.1073/pnas.84.12.4332. Proc Natl Acad Sci U S A. 1987. PMID: 3473504 Free PMC article.
-
An electron microscope study of the proteins attached to polio virus RNA and its replicative form (RF).Nucleic Acids Res. 1978 Dec;5(12):4711-23. doi: 10.1093/nar/5.12.4711. Nucleic Acids Res. 1978. PMID: 218168 Free PMC article.
-
Synthesis of a 600-nucleotide-long plus-strand DNA by virions of Moloney murine leukemia virus.Proc Natl Acad Sci U S A. 1979 Sep;76(9):4355-9. doi: 10.1073/pnas.76.9.4355. Proc Natl Acad Sci U S A. 1979. PMID: 92028 Free PMC article.
-
Structure of the provirus within NIH 3T3 cells transfected with Harvey sarcoma virus DNA.J Virol. 1981 Apr;38(1):125-35. doi: 10.1128/JVI.38.1.125-135.1981. J Virol. 1981. PMID: 7241646 Free PMC article.
-
The properties of native and denatured DNA in buoyant rubidium trichloroacetate at neutral pH.Nucleic Acids Res. 1977 Jun;4(6):1891-909. doi: 10.1093/nar/4.6.1891. Nucleic Acids Res. 1977. PMID: 19731 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
