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. 1983;2(1):57-61.
doi: 10.1002/j.1460-2075.1983.tb01380.x.

Cloning of several cDNA segments coding for human liver proteins

F Costanzo  1 , L Castagnoli, L Dente, P Arcari, M Smith, P Costanzo, G Raugei, P Izzo, T C Pietropaolo, L Bougueleret, F Cimino, F Salvatore, R Cortese
Affiliations

Cloning of several cDNA segments coding for human liver proteins

F Costanzo et al. EMBO J. 1983.

Abstract

A human cDNA library was constructed using M13 derivative vectors. The simple and rapid procedures for sequencing single-stranded DNA by the dideoxy chain termination method allowed a screening of individual clones directly by DNA sequence analysis. Some of these clones were identified as coding for: serum albumin, alpha1-antitrypsin, retinol-binding protein, prothrombin, haptoglobin, and metallothionein. Furthermore, a clone coding for aldolase B was tentatively identified on the basis of high sequence homology with rabbit muscle aldolase.

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References

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