doi: 10.1002/j.1460-2075.1983.tb01445.x.
The origin of replication, oriC, and the dnaA protein are dispensable in stable DNA replication (sdrA) mutants of Escherichia coli K-12
Affiliations
- PMID: 11894964
- PMCID: PMC555155
- DOI: 10.1002/j.1460-2075.1983.tb01445.x
Item in Clipboard
The origin of replication, oriC, and the dnaA protein are dispensable in stable DNA replication (sdrA) mutants of Escherichia coli K-12
EMBO J.
1983.
Abstract
The sdrA224 mutants of Escherichia coli K-12, capable of continued DNA replication in the absence of protein synthesis (stable DNA replication), tolerate inactivation of the dnaA gene by insertion of transposon Tn10. Furthermore, oriC, the origin of E. coli chromosome replication, can be deleted from the chromosome of sdrA mutants without loss of viability. The results suggest the presence of a second, normally repressed, initiation system for chromosome replication alternative to the 'normal' dnaA+ oriC+-dependent initiation mechanism.
Similar articles
-
dnaA suppressor (dasF) mutants of Escherichia coli are stable DNA replication (sdrA/rnh) mutants.Mol Gen Genet. 1984;196(2):350-5. doi: 10.1007/BF00328070. Mol Gen Genet. 1984. PMID: 6092872
-
The DnaA box R4 in the minimal oriC is dispensable for initiation of Escherichia coli chromosome replication.Nucleic Acids Res. 1995 Aug 25;23(16):3119-25. doi: 10.1093/nar/23.16.3119. Nucleic Acids Res. 1995. PMID: 7667087 Free PMC article.
-
The central lysine in the P-loop motif of the Escherichia coli DnaA protein is essential for initiating DNA replication from the chromosomal origin, oriC, and the F factor origin, oriS, but is dispensable for initiation from the P1 plasmid origin, oriR.Plasmid. 1998 Sep;40(2):91-9. doi: 10.1006/plas.1998.1349. Plasmid. 1998. PMID: 9735311
-
Research on DnaA in the early days.Res Microbiol. 2020 Dec;171(8):287-289. doi: 10.1016/j.resmic.2020.11.004. Epub 2020 Nov 25. Res Microbiol. 2020. PMID: 33245995 Review.
-
Regulating DNA replication in bacteria.Cold Spring Harb Perspect Biol. 2013 Apr 1;5(4):a012922. doi: 10.1101/cshperspect.a012922. Cold Spring Harb Perspect Biol. 2013. PMID: 23471435 Free PMC article. Review.
Cited by
-
Requirement of RecBC enzyme and an elevated level of activated RecA for induced stable DNA replication in Escherichia coli.J Bacteriol. 1990 Apr;172(4):1834-9. doi: 10.1128/jb.172.4.1834-1839.1990. J Bacteriol. 1990. PMID: 2180906 Free PMC article.
-
Effects of modulation of RNase H production on the recovery of DNA synthesis following UV-irradiation in Escherichia coli.Mol Gen Genet. 1987 Oct;209(3):494-8. doi: 10.1007/BF00331155. Mol Gen Genet. 1987. PMID: 2828881
-
Psi-Phi: exploring the outer limits of bacterial pseudogenes.Genome Res. 2004 Nov;14(11):2273-8. doi: 10.1101/gr.2925604. Epub 2004 Oct 12. Genome Res. 2004. PMID: 15479949 Free PMC article.
-
Stable DNA replication: interplay between DNA replication, homologous recombination, and transcription.Microbiol Mol Biol Rev. 1997 Jun;61(2):212-38. doi: 10.1128/mmbr.61.2.212-238.1997. Microbiol Mol Biol Rev. 1997. PMID: 9184011 Free PMC article. Review.
-
The absence of effect of gid or mioC transcription on the initiation of chromosomal replication in Escherichia coli.Proc Natl Acad Sci U S A. 1997 Nov 11;94(23):12497-502. doi: 10.1073/pnas.94.23.12497. Proc Natl Acad Sci U S A. 1997. PMID: 9356478 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
