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. 2002 Apr;46(4):1156-7.
doi: 10.1128/AAC.46.4.1156-1157.2002.

PCR-restriction fragment length polymorphism can also detect point mutation A2142C in the 23S rRNA gene, associated with Helicobacter pylori resistance to clarithromycin

Armelle Ménard, Adriana Santos, Francis Mégraud, Mónica Oleastro

PCR-restriction fragment length polymorphism can also detect point mutation A2142C in the 23S rRNA gene, associated with Helicobacter pylori resistance to clarithromycin

Armelle Ménard et al. Antimicrob Agents Chemother. 2002 Apr.
No abstract available

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Figures

FIG. 1.
FIG. 1.
Detection of mutation A2142C by BceAI-mediated restriction digestion. The restriction fragments of the 267-bp PCR products were analyzed by electrophoresis on a 5% agarose Resophor gel (A) or on a 12% polyacrylamide gel (B) stained with ethidium bromide. (A) PCR-RFLP analysis of mutations A2142G, A2143G, and A2142C occurring in domain V of the 23S rRNA gene of H. pylori. Lanes 1 and 8, 25-bp DNA Step Ladder molecular size markers (Promega). Lanes 2 and 3, PCR products of the wild-type and A2142G H. pylori strains digested with BbsI, respectively. Lanes 4 and 5, PCR products of the wild-type and A2143G H. pylori strains digested with BsaI, respectively. Lanes 6 and 7, PCR products of the wild-type and A2142C H. pylori strains digested with BceAI, respectively. (B) PCR product of the H. pylori strain with mutation A2142C digested with BceAI. Lanes 2 and 3, amplified wild-type PCR product and amplified PCR product presenting the A2142C mutation, respectively. Lane 1, 25-bp DNA Step Ladder (Promega). The wild-type H. pylori reference strain CIP 101260 and strains 683, 677, and 825, with mutations A2142G, A2142C, and A2143C, respectively, in the 23S rRNA gene were used as controls in this study (8).
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