Visual cortical organization at the single axon level: a beginning

Abstract

Single axon analysis of visual cortical connections is an important extension of previous anterograde studies using 3H-amino acids or wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP). The higher resolution tracers-Phaseolus vulgaris-leucoagglutinin (PHA-L), biocytin, biotinylated dextran amine (BDA) and dextran-conjugates-have already produced new results, simply by providing improved visualization, concerning laminar definition and possible subtypes of connections, as well as the beginning of a database of morphometrics and microstructure. The comparative approach, comparing geniculocortical terminations and cortical connections across several areas, has suggested both specific structural-functional correlations (for example, in extrastriate area MT/V5) and more subtle, possibly gradient-wise variations. Likely future directions for this line of research include more direct correlations of axon geometry with functional architectures, investigations of microcircuitry at the level of electron or confocal microscopy, anatomical and functional investigations of connectional convergence and interactions, and, not least, a more comprehensive database.