Wide-field electron microscopy. A rapid method for the study of histologic material that provides a bridge between light and electron microscopy

Abstract

Wide fields of tissue can be rapidly examined by electron microscopy by use of Formvar films for the support of ultrathin sections on slot grids. The intervention of the grid bars of conventional mesh grids is avoided, and a continuous micrograph of the specimen at scanning magnifications can be obtained. Enough material is sublimed from the section and the supporting film by deliberate exposure to the electron beam to permit one to obtain an image with good contrast. This method of examination, which takes in all about two hours, permits examination of an extensive area of tissue in relation both to its topography at low magnifications and to its ultrastructural detail, and accordingly adds to electron microscopy a dimension characteristic of the lower powers of the light microscope. It offers the histopathologist the option of using micrographs taken during the scanning survey of a tissue to detect regions that can be readily re-examined at high magnification in the same ultrathin section.