doi: 10.1099/00221287-115-2-517.
Preparation of protoplasts and whole cell ghosts from Mycobacterium smegmatis
- PMID: 119034
- DOI: 10.1099/00221287-115-2-517
Item in Clipboard
Preparation of protoplasts and whole cell ghosts from Mycobacterium smegmatis
J Gen Microbiol.
1979 Dec.
Abstract
Cell wall-deficient forms of Mycobacterium smegmatis were produced in growth medium containing D-cycloserine and horse serum. These cells were transformed into protoplasts with EDTA and lysozyme. Subsequent lysis by nucleases followed by osmotic shock produced membrane vesicles (whole cell ghosts).
Similar articles
-
Ultrastructural and chemical studies on wall-deficient forms, spheroplasts and membrane vesicles from Mycobacterium aurum.J Gen Microbiol. 1981 May;124(1):71-9. doi: 10.1099/00221287-124-1-71. J Gen Microbiol. 1981. PMID: 7033468
-
Protoplast formation of selected Mycobacterium smegmatis mutants by lysozyme in combination with methionine.J Bacteriol. 1977 Mar;129(3):1628-31. doi: 10.1128/jb.129.3.1628-1631.1977. J Bacteriol. 1977. PMID: 845126 Free PMC article.
-
[Spheroplasts of Mycobacteria. 1. Spheroplast formation with glycine and lysozyme].Igaku To Seibutsugaku. 1968 Jun;76(6):301-5. Igaku To Seibutsugaku. 1968. PMID: 4881607 Japanese. No abstract available.
-
Ultrastructural organization of spheroplasts induced in Mycobacterium sp. smegmatis by lysozyme or glycine.Arch Mikrobiol. 1969;69(3):227-36. doi: 10.1007/BF00408975. Arch Mikrobiol. 1969. PMID: 4195096 No abstract available.
-
Evidence for the synthesis of peptidoglycan by spheroplasts of Mycobacterium smegmatis ATCC-14468.Ann Microbiol (Paris). 1984 May-Jun;135A(3):367-74. doi: 10.1016/s0769-2609(84)80078-2. Ann Microbiol (Paris). 1984. PMID: 6465738
Cited by
-
Multiple drug resistance in Mycobacterium avium: is the wall architecture responsible for exclusion of antimicrobial agents?Antimicrob Agents Chemother. 1981 Nov;20(5):666-77. doi: 10.1128/AAC.20.5.666. Antimicrob Agents Chemother. 1981. PMID: 6798925 Free PMC article.
-
Gene manipulation in mycobacteria.Folia Microbiol (Praha). 1991;36(5):411-22. doi: 10.1007/BF02884059. Folia Microbiol (Praha). 1991. PMID: 1821865 Review.
-
Techniques for genetic engineering in mycobacteria. Alternative host strains, DNA-transfer systems and vectors.Antonie Van Leeuwenhoek. 1996 Apr;69(3):243-56. doi: 10.1007/BF00399613. Antonie Van Leeuwenhoek. 1996. PMID: 8740907 Review.
-
Spheroplast formation of Mycobacterium smegmatis and morphological aspects of their reversion to the bacillary form.J Bacteriol. 1982 Aug;151(2):1035-9. doi: 10.1128/jb.151.2.1035-1039.1982. J Bacteriol. 1982. PMID: 7096262 Free PMC article.
-
DivIVA concentrates mycobacterial cell envelope assembly for initiation and stabilization of polar growth.Cytoskeleton (Hoboken). 2018 Dec;75(12):498-507. doi: 10.1002/cm.21490. Epub 2018 Nov 30. Cytoskeleton (Hoboken). 2018. PMID: 30160378 Free PMC article.