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. 1979 Dec;115(2):517-21.
doi: 10.1099/00221287-115-2-517.

Preparation of protoplasts and whole cell ghosts from Mycobacterium smegmatis

N Rastogi, T A Venkitasubramanian

Preparation of protoplasts and whole cell ghosts from Mycobacterium smegmatis

N Rastogi et al. J Gen Microbiol. 1979 Dec.

Abstract

Cell wall-deficient forms of Mycobacterium smegmatis were produced in growth medium containing D-cycloserine and horse serum. These cells were transformed into protoplasts with EDTA and lysozyme. Subsequent lysis by nucleases followed by osmotic shock produced membrane vesicles (whole cell ghosts).

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