Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2002 Apr 5;296(5565):141-5.
doi: 10.1126/science.1071006. Epub 2002 Mar 21.

Functional annotation of a full-length Arabidopsis cDNA collection

Motoaki Seki  1 , Mari Narusaka, Asako Kamiya, Junko Ishida, Masakazu Satou, Tetsuya Sakurai, Maiko Nakajima, Akiko Enju, Kenji Akiyama, Youko Oono, Masami Muramatsu, Yoshihide Hayashizaki, Jun Kawai, Piero Carninci, Masayoshi Itoh, Yoshiyuki Ishii, Takahiro Arakawa, Kazuhiro Shibata, Akira Shinagawa, Kazuo Shinozaki
Affiliations

Functional annotation of a full-length Arabidopsis cDNA collection

Motoaki Seki et al. Science. .

Abstract

Full-length complementary DNAs (cDNAs) are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. We isolated 155,144 RIKEN Arabidopsis full-length (RAFL) cDNA clones. The 3'-end expressed sequence tags (ESTs) of 155,144 RAFL cDNAs were clustered into 14,668 nonredundant cDNA groups, about 60% of predicted genes. We also obtained 5' ESTs from 14,034 nonredundant cDNA groups and constructed a promoter database. The sequence database of the RAFL cDNAs is useful for promoter analysis and correct annotation of predicted transcription units and gene products. Furthermore, the full-length cDNAs are useful resources for analyses of the expression profiles, functions, and structures of plant proteins.

PubMed Disclaimer

Publication types