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. 2002 Apr;40(4):1390-4.
doi: 10.1128/JCM.40.4.1390-1394.2002.

Detection of a human rotavirus with G12 and P[9] specificity in Thailand

Y Pongsuwanna  1 , R Guntapong, M Chiwakul, R Tacharoenmuang, N Onvimala, M Wakuda, N Kobayashi, K Taniguchi
Affiliations

Detection of a human rotavirus with G12 and P[9] specificity in Thailand

Y Pongsuwanna et al. J Clin Microbiol. 2002 Apr.

Abstract

G12 rotavirus has not been detected anywhere in the world since the first detection of a human strain, L26 (G12, P1B[4]), in the Philippines in 1990. In this study, we isolated a human rotavirus (strain T152) with a VP7 of G12 specificity from the stool of an 11-month-old diarrheic patient in Thailand. The strain T152 exhibited a long RNA pattern and subgroup I specificity. In the comparison of the nucleotide and amino acid sequences of the VP7 gene of strain T152 with those of rotaviruses with different G type specificities, strain T152 showed the highest identity, 90.9 and 93.9%, respectively, to G12 prototype strain L26. In contrast, the VP4 gene of strain T152 showed the highest identity with P[9] specificity of human strains K8 and AU-1 and feline strains Cat2 and FRV-1, with homologies of 89.3 to 90.6% at the nucleotide level and 93.9 to 95.6% at the amino acid level. Thus, strain T152 was found to be a natural reassortant strain with G12 and P[9] specificities.

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Figures

FIG. 1.
FIG. 1.
RNA pattern of strain T152 in PAGE. Lane 1, KU; lane 2, L26; lane 3, T152.
FIG. 2.
FIG. 2.
Comparison of VP7 amino acid sequences from strains T152 and L26. Only the amino acids of strain L26 that are different from those of strain T152 are shown below the T152 amino acid sequence. Three major antigenic regions are boxed.
FIG. 3.
FIG. 3.
Phylogenetic tree for the nucleotide sequences of the VP8*-encoding region of the VP4 gene of human and feline rotaviruses. The bar indicates the variation scale.
See this image and copyright information in PMC

References

    1. Armah, G. E., C. T. Pager, R. H. Asma, F. R. Anto, A. B. Oduro, F. Binka, and D. Steele. 2001. Prevalence of unusual human rotavirus strains in Ghanaian children. J. Med. Virol. 63:67-71. - PubMed
    1. Estes, M. K. 1996. Rotaviruses and their replication, p. 1625-1656. In B. N. Fields, D. M. Knipe, P. M. Howley, R. M. Chanock, J. L. Melnick, T. P. Monath, B. Roizman, and S. E. Straus (ed.), Fields virology, 3rd ed. Lippincott-Raven Publishers, Philadelphia, Pa.
    1. Gentsch, J. R., P. A. Woods, M. Ramachandran, B. K. Das, J. P. Leite, A. Alfieri, R. Kumar, M. K. Bhan, and R. I. Glass. 1996. Review of G and P typing results from a global collection of strains: implications for vaccine development. J. Infect. Dis. 174(Suppl. 1):S30-S36. - PubMed
    1. Griffin, D. D., C. D. Kirkwood, U. D. Parasgar, P. A. Woods, J. S. Bresee, R. I. Glass, J. R. Gentsch, and The National Rotavirus Strain Surveillance System Collaborating Laboratories. 2000. Surveillance of rotavirus strains in the United States: identification of unusual strains. J. Clin. Microbiol. 38:2784-2787. - PMC - PubMed
    1. Kaga, E., M. Iizuka, T. Nakagomi, and O. Nakagomi. 1994. The distribution of G (VP7) and P (VP4) serotypes among human rotaviruses recovered from Japanese children with diarrhea. Microbiol. Immunol. 38:317-320. - PubMed

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