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. 2002 Mar;8(3):263-8.
doi: 10.3201/eid0803.010194.

Novel cryptosporidium genotypes in sporadic cryptosporidiosis cases: first report of human infections with a cervine genotype

Corinne S L Ong  1 , Diane L Eisler, Alireza Alikhani, Vicki W K Fung, Joan Tomblin, William R Bowie, Judith L Isaac-Renton
Affiliations

Novel cryptosporidium genotypes in sporadic cryptosporidiosis cases: first report of human infections with a cervine genotype

Corinne S L Ong et al. Emerg Infect Dis. 2002 Mar.

Abstract

In this study, we genotyped parasites from the fecal specimens of sporadic cryptosporidiosis cases in British Columbia from 1995 to 1999. Genotyping was conducted by polymerase chain amplification of the internal transcribed spacer region, a hypervariable region in the 18S rRNA gene and the Cryptosporidium oocyst wall protein gene. Subsequent analysis was by restriction fragment length polymorphism and DNA sequencing. We identified two new Cryptosporidium genotypes in humans. One of these genotypes has been found recently in deer in New York state. The other genotype has not been identified in humans or animals. These results have important implications for drinking water quality strategies, especially for communities that obtain drinking water supplies from surface sources located in forested regions with deer populations.

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Figures

Figure 1
Figure 1
Restriction profiles obtained after digestion of polymerase chain reaction products from the ITS1 locus with Mse I. lanes 1- 3-, 100-, 50-, and 10-bp ladder molecular weight markers; lanes 4 and 15, bovine genotype 2 isolates; lanes 5, 6, 14, and 16, human genotype 1 isolates including DE340 (lane 14); lanes 7 and 9–12, cervine genotype isolates including MH205 (lane 7), TK320 (lane 10), and DE302 (lane 11); lane 8, Cryptosporidium meleagridis isolate CS33; lanes 13 and 17, other novel genotype isolates such as VF383 (lane 13) and TK348 (lane 17)
Figure 2
Figure 2
Phylogenetic relationship of isolates from sporadic cases with reference 18S rRNA gene sequences from various Cryptosporidium species and genotypes. Bootstrap values that are >95% are shown in larger font. Bar = 0.01 substitution per site.
Figure 3
Figure 3
Restriction profiles obtained after digestion of polymerase chain reaction products from the Cryptosporidium oocyst wall protein locus with Rsa I. Lanes 1- 3-, 100-, 50-, and 10-bp ladder molecular weight markers; lanes 4 and 6, bovine genotype 2 isolates; lanes 7 and 8, human genotype 1 isolates; lane 9, cervine genotype isolate MH205; and lane 10, C. meleagridis isolate CS33
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