Pathogenicity of Australian strains of avian infectious bronchitis virus

Abstract

The pathogenicity of 25 strains of infectious bronchitis virus (IBV) isolated in Australia between 1961 and 1994 was compared in white leghorn specific pathogen-free chicks. Twelve strains were nephropathogenic and 10 respiratory, the other three being of mixed pathogenicity. The IBV strains identified as nephropathogenic induced clinical nephritis, gross and histological kidney lesions, and mortality of 5-90%. According to the severity of these features, the nephropathogenic strains could be further subdivided into strains of high, moderate or low pathogenicity. The three strains of mixed pathogenicity induced tracheitis, mild clinical nephritis and kidney lesions but no mortality. The 10 respiratory strains caused histological lesions in the trachea but not in the kidney, and did not induce clinical nephritis or mortality. Of 12 IBV strains isolated between 1961 and 1976, nine were nephropathogenic, inducing mortality of 15-90%. In contrast, of 13 strains isolated between 1981 and 1994, only three were nephropathogenic, inducing mortality of 5-37%, whereas nine were respiratory. Seven of these nine strains, unlike other respiratory strains, failed completely to replicate in the kidney. The results indicated a change in the prevalent IBV strains from highly nephropathogenic (1960s to 1970s) to respiratory (1980s to early 1990s); moreover, the late 1980s saw the emergence of respiratory strains with altered tissue tropism.